Transcript Developmental Biology 8/e - Florida International University

Neural Crest Cells
The cells of the neural crest migrate to generate many cell
types:
1) the neurons and glial cells of the sensory, sympathetic and
parasympathetic nervous systems;
2) the epinephrine-producing cell of the adrenal gland;
3) the melanocytes;
4) and many of the skeletal and connective tissue
components of the head.
13.1 Schematic representation of neural crest formation in an amniote embryo, cross section (1)
13.1 Schematic representation of neural crest formation in an amniote embryo, cross section (2)
13.2 Regions of the chick neural crest
13.3 Neural crest cell migration in the trunk of the chick embryo (Part 1)
13.3 Neural crest cell migration in the trunk of the chick embryo (Part 2)
2-day chick embryo stained
red with antibody to HNK-1
which selectively recognizes
NC cells. Extensive staining is
seen in the anterior part of
each sclerotome.
13.3 Neural crest cell migration in the trunk of the chick embryo (Part 3)
Cross sections through the chick embryo showing extensive migration
through the anterior portion of the sclerotome (C), but (D) no migration
through the posterior portion.
13.4 All migrating neural crest cells are stained red by antibody to HNK-1
• What signals initiate migration?
• When does the migratory agent become competent to respond to
these signals?
• How do the migratory agents know the route to travel?
• What signals indicate that the destination has been reached?
Wnt and FGF
BMP4 and BMP7
Slug and RhoB
13.5 Segmental restriction of neural crest cells and motor neurons by the ephrin
proteins of the sclerotome (Part 1)
13.5 Segmental restriction of neural crest cells and motor neurons by the ephrin
proteins of the sclerotome
13.6 Hair follicle of a mouse
Some of these melanocytes
migrate outside the bulge to
differentiate into mature
melanocytes and provide
pigmentation to the hair shaft.
13.7 Pluripotency of trunk neural crest cells (Part 1)
13.7 Pluripotency of trunk neural crest cells (Part 2)
13.8 Paracrine factors encountered in the environment help specify the different neural
crest-derived lineages in the trunk
13.9 Cranial neural crest cell migration in the mammalian head (Part 1)
13.9 Cranial neural crest cell migration in the mammalian head (Part 2)
Contributes to the forehead, nose,
philtrum of the upper lip, and to the
primary palate.
Generates the sides of the nose.
Lower and upper jaw, and to the sides of the
middle and lower regions of the face.
13.9 Cranial neural crest cell migration in the mammalian head (Part 3)
13.12 Intramembranous ossification (Part 1)
13.12 Intramembranous ossification (Part 2)
BMPs
CBFA1
Osteocalcin
Osteoporin
and others
Blue: cartilage
Red: bone
13.13 Cranial neural crest cells in embryonic mice, stained for -galactosidase expression (Part 1)
A, B, C: day 6-9.5
13.13 Cranial neural crest cells in embryonic mice, stained for -galactosidase expression (Part 2)
13.13 Cranial neural crest cells in embryonic mice, stained for -galactosidase expression (Part 3)
13.14 The septa that separate the truncus arteriosus into the pulmonary artery and
aorta form from cells of the cardiac neural crest (Part 1)
13.14 The septa that separate the truncus arteriosus into the pulmonary artery and
aorta form from cells of the cardiac neural crest (Part 2)
13.15 Cranial placodes form sensory neurons (Part 1)