Transcript Supplementary Figure 4

Supplementary figure 4
a
b
Pixels / 2h1/2
Cell movement
150
100
***
50
0
-50
-100
-150
-200
-250
c
e
Control
Netrin1
Netrin1+
UNC5B-Fc
d
Supplementary figure 4. Aortic ring assays.
a: overview of the culture. Scale bar, 0.5cm.
b: quantification of cell movement. The distance of cell
movement (pixel/2h1/2) was measured for each cell filmed
(n=6 control, 8 Netrin-1, 16 Netrin-1/UNC5B-Fc).
The mean±s.e.m. of all cells for each treatment is plotted.
Reverse movement is denoted -.
c: see movie1, control endothelial cells.
d: see movie2, cells exposed to a gradient of pre-clustered
Netrin-1/UNC5B-Fc (arrow). Movement of the
two leading cells was quantified.
e: see movie3, two cells exposed to a gradient of Netrin-1.
Note retraction (movie3).