Transcript Oleksandr Veselov

Oleksandr Veselov
Henryk Niewodniczański Institute of Nuclear Physics
Polish Academy of Sciences
Krakow, Poland
Single Ion Hit Facility
on-line imaging
beam off
signal
position
control secondary
target
setup
electrons
detector
accelerator VdG
single ion
detection
beam off
optical setup
slits
measurement
chamber
exit
focusing
window
quadrupoles
• to image
• to recognize
• to place
• to hit
deflection
high
plates
precision
slits
proton
beam
 to place cell
 to turn on the beam
 to wait for signal
 to turn off the beam
 to place next cell
To image: Quantitative Phase microscopy (QPm)
 The observation of living cells in their native state (if possible without staining)



k z I r     I r  r 
- equation
 The Transport of Intensity Equation (Teague, 1983)

1

2
  k          z I   - solution
I


2

Transparent
sample
Detector
increase in
intensity
decrease in
intensity
increase in
intensity
Collimated
light
Phase (wave)
front
The processing of 1.31 megapixel image
(1280x1024 pixels, 480x385 µm2) took about
5 seconds using Athlon XP 2800+ PC
QPm Results
brightfield image
QPm image
brightfield image
QPm image
To recognize: Opening
The opening of a binary image I by a structuring element B can be expressed as
the union of all translates of B that fit inside I . It effectively removes any image
components which cannot completely `hold' the structuring element.
Opening smoothes object contours, separates narrowly connected objects and
removes small objects
Opening: Example
original QPm image
after binarization
opening applied 5 times
opening applied 7 times
Recognition: Area
Number of cells
40
Cell area distribution
30
20
10
0
0.0
0.5
1.0
1.5
2.0
Normalized cell area
2.5
100mm
Positioning: CR-39
100mm
Future Plans



To finish the positioning
To be able to accumulate a set of images in the
multiple scan area and to combine them into a
single, “tiled” image
To develop contrast enhancing and cell recognition
methods