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BSC 197: Molecular and Cellular Basis of Life Lab TA info • • • • Drinie Clairardin [email protected] Office/Lab: SLB 245/247 http://biology197.wikispaces.com/ Lab Info • Lab schedule can be found on the syllabus – Course website: www.bio.ilstu.edu/bsc197 • Lab will consist of an exercise one day and a wet lab the following day Lab Safety • Safety is the number one priority • ABSOLUTELY NO food, drink or tobacco in Lab • Lab safety equipment available and will be used as needed (eg. Goggles, gloves) • Location of Eyewash, fire extinguisher • Do not endanger yourself or others • Respect everyone in lab Assignments • All lab reports must be typed (including graphs) • Do the lab report as follows – Intro: Short background about the lab; include hypothesis – Materials and Methods: Summarize briefly; focus on the reasons, don’t need exact details – Results: tables/graphs of data and text to summarize what they show – Discussion: analysis of results; troubleshoot lab if it didn’t work – Conclusion: short summary of everything • All lab reports are due at the beginning of the lab, two days after the lab was completed Plagiarism Policy • Plagiarism will not be tolerated – Consequences of being caught for plagiarism include receiving a zero for the class and being referred to student affairs for disciplinary action • Do your own work – Make your own graphs – Write your own papers – Cite sources Exercise 1 • We will use several types of graphs in the lab – Bar (listed as column graph in excel): good choice when only using a few data points; can have words in the x-axis; x-axis is not evenly numbered Graphs (cont’d) • Line Graph: used when you are looking at a change over time; x-axis not drawn to scale and x-axis can have letters Graphs (cont’d) • Scatter (XY Scatter): X-axis drawn to scale; data will be plotted in its exact position not just at the labeled point; can not be used if there are letters needed on the x-axis Graphs (cont’d) • Pie chart: Used when describing a part or percentage of a whole Graphs (cont’d) • Graph requirements: – X and Y axis titles, labels and units – Graph title (must be descriptive) – Legend – Failure to include ALL requirements will result in point deductions Statistical Calculations • Pages 11-14 (top portion-not including T-Test) • Mean- Average X 1 X 2 X 3 X 4 X 5... MEAN N •N = total number of replicates •X1, X2, X3… Individual data Points Mean Calculation Example • • • • • • X1 = 79 X2 = 85 X3 = 92 X4 = 87 X5 = 93 X6 = 99 • (79 + 85 + 92 + 87 + 93 + 99)=535 • 535/6=89.17 Statistical Calculations (cont’d) • Standard Deviation (S.D.)- measures variability of the mean S.D. N *(X ) (X ) N ( N 1) 2 2 •N- number of replicates •(X2)- Sum of the squares of X •(X)2- The square of the sum of the values for X Standard Deviation (S.D.) Example Calculation Larva # X Larva # X X2 1 0.124 1 0.124 0.015376 2 0.11 2 0.11 0.0121 3 0.131 3 0.131 0.017161 4 0.099 4 0.099 0.009801 5 0.119 5 0.119 0.014161 N=5 Sum of X = 0.583 Sum of X2 = 0.068599 Square X values 5(0.068599) - ((0.583) 2 ) S.D. 5(5 1) S.D.= 0.012461942 Statistical Calculations (cont’d) • Standard Error (S.E.): estimates reliability of the mean ( S.D.) S . E. (N ) (0.012461942) S . E. 5 S.E.= 0.005573149 = 0.006 (rounded off) Statistical Calculations (cont’d) • Do not round values until the end • Show all your work for the calculations For tomorrow: • Exercise 1 due • Lab 1: The Properties of Enzymes – Read Lab 1 in the manual • Dress appropriately – No open toed shoes or shorts above the knee!