Transcript PPT1
Motor mechanisms Keywords (reading p. 10801086) • • • • • • • • Bundle, fiber, myofibril, sarcomere Z-line, thick filament, thin filament Actin, myosin, sliding filament model Molecular basis for filament movement Troponin, tropomyosin Sarcoplasmic reticulum Integration of synaptic signals neurotransmitters Motor mechanisms • Create movement • Can be cilia, flagella, contractile proteins, muscles • Will focus on skeletal muscle – Muscle of vertebrates that is under voluntary control Structure of skeletal muscle • • • • Bundle Fiber Myofibril sarcomere Part of Campbell 6th ed p. 1081 Features of muscle cells • # of nuclei- multinucleate; formed by fusion of embryonic cells • length - this results in very long cells Part of Campbell 6th ed p. 1081 Sarcomere • Structure gives muscle a striated appearance • Z line, thick filaments (myosin), thin filaments (actin) Part of Campbell 6th ed p. 1081 Part of Campbell 6th ed p. 1081 Sliding-filament model of muscle Part of Campbell 6th ed contraction p. 1081 Thick and thin filaments slide Part of Campbell 6th ed past each otherp. 1081 At maximal contraction, there is no space at end of thick filament, thin filaments overlap Part of Campbell 6th ed p. 1081 http://www.youtube.com/watch?v=InIha7bCTjM&feature=related Molecular basis for movement of filaments against each other Part of Campbell 6th ed p. 1082 ATP bound, head retracted and unattached Part of Campbell 6th ed p. 1082 Hydrolysis of ATP cocks head Part of Campbell 6th ed p. 1082 Myosin head attaches to actin filament Part of Campbell 6th ed p. 1082 Release of ADP + Pi causes a further conformational change pushing against the actin filament Part of Campbell 6th ed p. 1082 Binding of ATP to myosin head causes dissociation from actin filament Part of Campbell 6th ed p. 1082 Cycle repeats and sarcomere shortens Part of Campbell 6th ed p. 1082 Control of muscle contraction by Ca++ • Tropomyosin- blocks the myosin binding sites on the actin filament when muscle is at rest • Troponin complex-binds calcium and controls the position of tropomyosin At rest, myosin cannot bind because sites are covered by tropomyosin http://www.youtube.com/watch?v=WRxsOMenNQM Part of Campbell 6th ed p. 1083 During muscle contraction Ca++ ++ levels rise. Ca binds to troponin which then pulls tropomyosin way from the binding sites Part of Campbell 6th ed p. 1083 What triggers the Ca++ rise that induces muscle contraction? [Ca++] regulated by the sarcoplasmic reticulum Campbell 6th ed p. 1083 Structure of the sarcoplasmic reticulum • T tubules - are a network of the fiber plasma membrane that goes deep into the muscle fiber. • This allows transmission of the action potential into the fiber Sequence of events leading to muscle contraction • Motor neuron releases acetylcholine • Depolarization of the muscle fiber membrane results in action potentials • Action potentials trigger release of Ca++ from the sarcoplasmic reticulum • Increased Ca++ allows actin and myosin to slide against each other Characteristics of other muscle types: • Cardiac muscle - found only in the heart, striated, gap junctions allow direct electrical signaling between cells • Smooth muscle - involuntary muscle, meshwork of actin and myosin, can contract more (greater shortening), but with less tension.