Transcript Aeromonas hydrophila

Aeromonas hydrophila
Jennifer Palmer
Carolinas College of Health Science
School of Clinical Laboratory Sciences
July 12, 2010
Objectives
After completion of the presentation, the participant
will:
Identify the causative agent, Aeromonas hydrophila by
biochemical reactions, growth requirements and colony
morphology.
Describe the symptoms associated with an Aeromonas
hydrophila infection.
Understand the route of transmission and the populations
affected.
Interpret how this infection is diagnosed.
Evaluate the prognosis of the infection.
Explain the risk factors and prevention of the infection.
Etiologic Agent
Aeromonas hydrophila
Facultative anaerobe
Gram negative rod
Motile with polar flagella
Beta hemolytic on BAP
Tolerates temperatures from
4-42oC
Humans and fish are known
hosts
Resistant to chlorine
http://www.buddycom.com/bacteria/gnr/gnrgluox.html
http://www.microbiologyatlas.kvl.dk/biologi/showmorf.asp?articleid=4
Route of Transmission
Waterborne- distributed in
freshwater and marine
environments all over the world. *
Reaches a peak in the Summer
months when the water
temperatures are warmer. *
May be transmitted from eating
infected seafood, meat, or even
sprouts.
Transmitted when a person enters
contaminated water with an open
wound.
Also, drinking the contaminated
water.
http://www.blog.thesietch.org/2009/03/23/ibm-rolls-out-more-energy-efficient-water-purification/
Symptoms
May range from
wound infections to
septicemia to
gastroenteritis. *
Wound infections
caused by Aeromonas
hydrophila can be
classified into 2
categories: cellulitis
and myonecrosis.
http://www.rd.com/clean-jokes-and-laughs/silly-docotor-cartoons/article116871-5.html
Cellulitis and Myonecrosis
http://web.mst.edu/~microbio/BIO221_2007/A_hydrophiila_2.htm
Gastroenteritis
There may be two
forms: a disease similar
to traveler’s diarrhea,
and dysenteric
gastroenteritis.
The dysenteric diarrhea
will have blood and
mucus, and may last
for days to weeks.
http://helpfulhealthtips.com/Images/D/diarrhea1.jpg
Diagnosis
Can be diagnosed by a combination
of the Gram stain, and a myriad of
biochemical reactions.
Also, the patient’s history and
presenting symptoms will help the
doctor in diagnosis.
http://www.stopdizziness.com/patients_billing.asp
Biochemical Reactions
KIA/TSI=K/Ag, Oxidase
POSITIVE!
H2S positive
ONPG positive
Indole positive
Nitrate positive
Gel Positive
DNase positive
BE positive
Ornithine positive
http://bodylogicmd.files.wordpress.com/2009/11/1074test_tubes1.jpg
Prognosis
If immunocompetent, the mild gastroenteritis is
usually self-clearing.
In immunocompromised people who have the
dysenteric diarrhea may need a course of
antibiotics, and fluids.
In the wound infections, antibiotics may need
to be administered and the wound needs to be
kept very clean.
Risk Factors
This is an opportunistic
pathogen so the
population affected is the
immunocompromised.
Most people who are
affected by Aeromonas
have cirrhosis, or other
predisposing disease.*
Occurs all over the
world, mainly in coastal
cities, and all ages can
be affected.
http://www.hawaiimagazine.com/images/content/two_Hawaii_Dr_Beach_top_ten/Hamoa%20Beach.jpg
Treatment
The drugs of choice are
2nd and 3rd generation
Cephalosporins, and
Aminoglycosides.*
Has resistance to
Penicillin, Ampicillin,
Carbenicillin, and
Ticarcillin. *
http://online.pillslib.com/cefdinir/cefdinir-medicine
Prevention
Take proper care of
wounds, washing with
sterile water, not river
water.
May be killed using 1%
sodium hypochlorite and
two percent calcium
hypochlorite solution.
http://apptemp.houstonisd.org/procurement//WarehouseCatalog1/PictureCatalog.aspx
References
Book sources:
Mahon, Connie, Donald Lehman, and George Manuselis. Textbook of Diagnostic
Microbiology. St. Louis: Saunders, 2007
Gantz, et al.. Manual of clinical problems in infectious disease. Philadelphia:
Lippincott, Williams &Wilkins, 2006.
Internet sources:
Guntly, Lisa. "Microbe of the week 2007". Missouri S&T biology department.
Accessed 06/27/2010
<http://web.mst.edu/~microbio/bio221_2007/a_hydrophiila_2.htm
"BBB-Aeromonas hydrophila". USFDA. Accessed 06/27/2010
<http://www.fda.Gov/food/FoodSafety/FoodborneIllness/Foodborn
eIllnessFoodbornePathogensNaturalToxins/BadBugBook/ucm070523
.htm>.
Journal source*:
Chang, et al.. "Recurrent dialysis-associated Aeromonas hydrophila
peritonitis: reports of two cases and review of the literature".
Peritoneal dialysis international 2005: 496-502.
http://outlookportland.wordpress.com/2009/10/