Transcript Alternative Design

Inhalation System for Animal
Infection
With BSL3 Pathogens
Team Members:
Stacey Hoebel
Maja Middleton
Alternative Design:
One-Compartment Box

Modification of current lab design
– Safer and more efficient

One large compartment made of
plastic able to withstand autoclave
– No need for individual compartments for
animals
– Small enough to fit under fume hood
Alternative Design:
One-Compartment Box


Tube from nebulizer
into compartment
to administer
pathogen
Tube from
compartment to
ethanol trap and
HEPA filter
Alternative Design:
One-Compartment Box
Pros
 Very inexpensive
 Easy to
manufacture
 Better quality
– Plastic compartment
– Seal around tubing
and lid
Cons
 Too simple
 Doesn’t address all
problems
– Contaminates entire
body of animal
– Inconsistent amount
of infection
administered to
animals
Alternative Design:
Central Porous Tubing

Plastic box with lid
– Split into six built-in compartments which hold one animal
each

Central Porous Tubing
– One tube runs from nebulizer then down middle of box,
between compartments
– Infectious gas expelled out of holes in tubing into
individual chambers
– Remaining gas led to HEPA filter and ethanol trap

Restraint Device
– Holds animal in place while being infected
Alternative Design:
Central Porous Tubing
Alternative Design:
Central Porous Tubing
Pros
 Individual
compartments and
restraints keep animals
separated, calm
 Better control of agent
 Relatively inexpensive
 Easy to manufacture
Cons
 Not accurate enough
– Impossible to regulate
how much agent is
administered to each
animal
Final Design:
Multi-Chamber w/ Nose Piece

Plastic box with lid
– Made of polymethyl
pentene
(autoclavable)
– Split into four builtin compartments

Moveable back wall
to adjust to animals
of various sizes
– Small enough to fit
under fume hood
Final Design:
Multi-Chamber w/ Nose Piece

Split Tubing
– Nebulizer leads into one
tube
– Router splits this tube
into four separate tubes
– Each tube leads to a
different compartment
– Another router
condenses tubing into
one tube leading to
ethanol trap and HEPA
filter
Final Design:
Multi-Chamber w/ Nose Piece

Neck Seal
– “Wall” of stretchy
material set 1.5 inches
from front of
compartment
– Small hole in center of
material for head and
neck
– Holds animal in place
– Creates seal between
head and body
Final Design:
Multi-Chamber w/ Nose Piece

Nose piece
– Cone formed at end
of tubing by
snipping each side
and caulking
between cut
– Does not need to be
directly attached to
head
– Directs airflow to
nose and mouth
Final Design:
Multi-Chamber w/ Nose Piece
Pros
 Separate tube for each chamber
– Consistent, efficient administration of pathogen

Minimization of body contamination
– Nose piece directs airflow
– Seal between head and body

Able to sanitize entire system

Many safeguards to prevent leakage of agent
Final Design:
Multi-Chamber w/ Nose Piece
Future Work
 Consult client about possibly
anesthetizing animals
– Complicated to monitor sedation levels
– Could lose some animal subjects

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Organization system for all the tubing
Appropriate materials (able to sterilize,
non-porous)
Questions?