Transcript EP & TA Laboratories and Veterinary Diagnostics LTD

EP & TA Laboratories and Veterinary
Diagnostics Ltd
16 Phalirou Str. Ayios Pavlos 2362
Nicosia – Cyprus
Tel. No. 00357 22 444 230
Fax No. 00357 22 444 231
Email: [email protected]
EP & TA Laboratories ORGANOGRAM
• Dr Pavlos Economides D.V.M.-F.R.S.H
Veterinarian-Bacteriologist – Director
• Dr Savvas Gennaris D.V.M.-Veterinarian
• Dr Marios Christoforou DVM-Msc –
Veterinarian-Associate
• Mr Tefkros Anaxagorou – Quality ManagerChief Technician
• Mrs Alkisti Gennari – Chemical Engineer
• Mr Anaxagoras Anaxagorou – Electrical
Engineer-Laboratory Technician
• Mr Anaxagorou Andreas - Sampling
EP & TA Laboratories and Veterinary Diagnostics
Activities
VETERINARY DIAGNOSTICS
-Microbiology
-Parasitology
-Serology
FOOD ANALYSIS
-Bacteriology
-Sensory Testing
-Shelf Life Testing
-Residues of inhibitory substances
TYPES OF SAMPLES EXAMINED
•Animal Tissues – Blood samples
•Foods of animal origin
•Meat and meat products
•Milk and milk products
•Fish and fish products
•Animal feeds
•Water
FOOD ESTABLISMENT HYGIENE
• Water supply – Bacteriological examinationsChlorine residues
• Evaluation of working surface Hygiene – Swab
tests
• Air samples - Bacteriology
METHODS FOR MICROBIOLOGICAL TESTING OF
FOODS
• Salmonellae ISO 6579-2002-Isolation –
Identification - Serotyping
• Escherichia Coli ISO 21528-2:2004
• Staphylococcus aureus ISO 6888-1:2003
• Listeria monocytogenes - Isolation – Identification
ISO 11290-2:1998
• Colony-count- enumeration of microorganisms in
animal feeding stuffs ISO 4833:2003
• Detection of Staphylococcal Enterotoxin in milk
products – ELISA –Transia Kit
METHODS FOR MICROBIOLOGICAL
EXAMINATIONS OF WATER
• Detection and enumeration of Escherichia coli
and Coliforms bacteria-Membrane filtration
method ISO 9308-1
• Detection and enumeration of intestinal
enterococci ISO 7899-2
• Total colony count ISO 6222
• Detection and enumeration of Faecal
Coliforms ISO 9308-1
• Detection of Legionella spp ISO 11731
METHODS FOR MICROBIOLOGICAL
TESTING OF SURFACES AND AIR SAMPLES
SAMPLING
TESTING
Rodac-contact plates
Hygicult Total Plate Count Agar
&
Hygicult E-Violet Red Bile Agar
with addition of glucose
• Total Plate Count ISO
4833:2003
• Detection and enumeration
of Enterobacteriaceae ISO
21528-2:2004
• Detection of Salmonella spp
ISO 6579-2002
• Detection and Enumeration
of Listeria monocytogenes
ISO 11290:1996
Bacteriological Examination
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Cultures
Identification of Bacteria – Biochemical testing
P.C.R.-Polymerase Chain Reaction
Immunofluorescence
Polymerase Chain Reaction –
Immunofluorescence Examination
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PCR
Legionella spp
Salmonellae spp
Listeria spp
Rickettsia
Coxiella
Leishmania
Bartonella
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Immunofluorescence
Legionella spp
Rickettsia
Coxiella
Leishmania
Bartonella
SEROLOGICAL TESTING
• Standard Agglutination Tests
• Complement Fixation Test
• ELISA Tests
ELISA TESTS
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ELISA IN POULTRY
Infectious Bursal Disease
– IBD
Infectious Bronchitis Virus
– IBV
Newcastle Disease Virus –
NDV
Avian Pneumovirus – TRT
Reovirus - REO
Mycoplasma
gallisepticum - MG
ELISA IN PIGS
• Reproductive and
Respiratory Syndrome –
PRRS
• Swine Influenza
• Aujeszky Disease
• Mycoplasma
Hyopneumoniae
• Porcine Parvovirus
• Transmissible
Gastroenteritis - TGE
• Corona Virus
PARASITOLOGICAL TESTING
• Microscopical examination
• Parasites Egg Count by Floatation –
McMaster
• Protozoa
CUSTOMERS
• Number of Farms
43
• Number of Establishments
78
Types of Farms
• Dairy Farms
• Pig Farms
• Poultry Farms
• Rabbit Farms
• Pets-dogs-cats
• Cage birds
Types of Establishments
• Poultry Slaughter houses
• Slaughter houses for pigs, sheep and
goats
• Food establishments
• Meat Cutting Plants
• Meat Products
• Dairies
• Butcheries
• Hotels
• Restaurants
SALMONELLAE SEROTYPES
ISOLATED
List of most common Salmonellae serotypes
isolated
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Typhimurium
Braenderup
Brandenburg
Newport
Hadar
Derby
Anatum
LISTERIA SEROTYPES ISOLATED
List of most common Listeria serotypes
isolated
• Listeria monocytogenes
• Listeria innocua
• Listeria welshimeri
SURVEILLANCE FOR ZOONOSES
• Brucellosis
• Rickettsioses
• Coxiella
• Bartonellosis
• Leishmaniosis
• Ehrlichiosis
COLLABORATION WITH OTHER INSTITUTIONS
• World Health Organization – WHO network SalmSurv as a General Institution Member for the GSS
country Databank
• University of Crete – School of Medicine - Laboratory
of Clinical Bacteriology-Parasitology-Zoonoses and
Geographic Medicine
• WHO Collaborating Center for Research and Training
in Mediterranean Zoonoses – Anogia - Crete
• Royal Veterinary College London – Department of
Pathology of Infectious Diseases - UK
PARTICIPATION IN INTERLABORATORY
TESTS
• We participate in all Interlaboratory testing
organized by the National Reference
Laboratory for foods of Animal Origin
July 2004 – Total Colony Count in
(accuracy
d=-0,03)
October 2004 - Total Colony Count in
(accuracy
d=-0,022)
September 2005 – Detection of Salmonella
cheese samples (accuracy
100%)
May 2006 - Total Colony Count in
(accuracy
d=-0,009)
milk samples
milk samples
and Listeria in
milk samples
APPROVAL - LICENCED
EP & TA Laboratories and Veterinary Diagnostics is
approved and licensed by
• The Department of Veterinary Services of Cyprus
• The Veterinary Council
The diagnosis tests of animal diseases, zoonotic agents
and for the bacteriological examination of foods of
animal origin including animal feeds. We are in the
process for accreditation in accordance with the
requirements of the ISO 17025:2005 standard.