Choose Bloodborne Pathogen and Biomedical Waste Training
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Transcript Choose Bloodborne Pathogen and Biomedical Waste Training
Biological Safety Office
Environmental Health & Safety
352-392-1591
www.ehs.ufl.edu
[email protected]
Sharon Judge, PhD
Assistant Biosafety Officer
Pathogenic microorganisms present in blood and other
potentially infectious material (OPIM) that are able to
cause disease in humans
Hepatitis B virus (HBV, HepB)
Hepatitis C virus (HCV, HepC)
Human immunodeficiency virus (HIV)
Less commonly, human T-lymphotropic virus (HTLV-1),
Epstein-Barr virus (EBV), malaria, brucellosis, rabies,
leptospirosis, babesiosis, syphilis, Creutzfeld-Jakob
disease, arboviral infections (WNV, EEE), etc.
Implemented in 1991 by the Occupational Safety &
Health Administration (OSHA)
29 CFR 1910.1030
http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051
Revised in 2001 – safe sharps devices, maintain a log of
injuries from contaminated sharps
UF follows OSHA requirement
General and workplace-specific training
Completed BEFORE individual is assigned to tasks with the
potential for BBP exposure and ANNUALLY thereafter
In addition to training, individuals with potential exposure
must also have:
Access to the regulatory text and an explanation of its contents
http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051
Access to a copy of the UF Exposure Control Plan
http://www.ehs.ufl.edu/Bio/BBP/ECP2011.pdf
Access to site-specific Standard Operating Procedures (SOPs)
http://www.ehs.ufl.edu/Bio/BBP/BBPSOPS.pdf
Chairs/Directors
Ensure dept. compliance
Faculty/Supervisors
Ensure appropriate exposure control plan is in place and being
followed
Employees, students, volunteers, etc
Follow exposure control plan, report problems/exposure
SHCC/Occ. Med
Immunizations & post-exposure follow-up
EH&S Biosafety
Develop/coordinate program, track participants
ALL employees, staff, students, volunteers, affiliates with
potential exposure to BBP from human blood/OPIM
Custodians, medical providers, dentists/dental staff,
autopsy staff, clinical laboratory staff, research lab staff
& students, biomedical engineers, athletic trainers,
event staff, police, emergency responders, physical
plant workers…etc
YES
NO*
Cerebrospinal fluid
Tears
Synovial fluid
Feces
Peritoneal fluid
Urine
Pericardial fluid
Saliva
Pleural fluid
Nasal secretions
Semen/Vaginal secretions
Sputum
Breast milk
Sweat
Amniotic fluid
Vomit
*unless visibly contaminated
with blood
1. Cuts or punctures with contaminated sharp objects
2. Splashes to mucous membranes
3. Contamination of broken/non-intact skin
A woman in KY was arrested and charged with public
intoxication (March 2010)
While changing into an inmate uniform, she squirted a
stream of breast milk into the face of a female deputy
The press release sparked a debate when it was noted
that the deputy was able to “clean the biohazard off of
her”
Does this constitute an occupational exposure?
Yes, breast milk is considered OPIM
All human blood or OPIM is treated
as infectious
Use:
Safety Equipment
Safe Work Practices
Personal Protective Equipment
(PPE)
Standard precautions = universal
precautions + body substance
isolation. Applies to blood & all other
body fluids, secretions, excretions
(except sweat), nonintact skin, and
mucous membranes
Human blood and OPIM
Objects/items contaminated by blood or OPIM
Unfixed human tissues/organs (other than intact
skin)
Cell or tissue cultures that may contain BBP agents
Blood/tissues from animals infected with BBP agents
Use Universal Precautions for all human cell lines
ATCC started testing newly manufactured/deposited cell
lines for common viral pathogens (HIV, HepB, HepC,
HPV, EBV, and CMV) in January 2010
Many infectious agents yet to be discovered and for
which there is no test
Remember HIV?
What about XMRV?
Spread through direct contact with infected
fluids (blood, semen, vaginal fluids)
Infection may be acute or chronic
body
~4.3-5.6% of Americans have been infected with HepB
5-10 % of adults will develop chronic infection; ~1.2 million people
with chronic HBV
15-25% develop cirrhosis , liver failure, or liver cancer (~ 3000
deaths/year)
Many people (~50%) are asymptomatic; if symptoms
occur they include:
Fever
Abdominal pain
Fatigue
Loss of appetite
Nausea
Vomiting
Jaundice
Joint pain
Dark urine
Percutaneous
~30% of these exposures results in infection
Mucosal exposure to blood/body fluids
Exposure to nonintact skin from contaminated surfaces
and equipment – HBV can remain infective in dried
blood at RT for at least 1 week (MacCannell et al., Clin Liver Dis
2010; 14:23-36)
Get vaccinated!
Universal Precautions
Cleaning/disinfection
Safe
Effective
Given to newborns, 120 million people
in U.S. have received at least one dose
>95% develop immunity after full series
(3 doses given at 0, 1, 6 mos)
In Gainesville, free @UF SHCC
(392-0627)
Bring completed
Acceptance/Declination statement with
you
http://www.ehs.ufl.edu/Bio/BBP/TNV.pdf
If you decline, can change mind at any
time
Health-care workers or public safety workers at high risk
for continued percutaneous or mucosal exposure to blood
or body fluids, HBV research lab workers
Performed 1-2 months after dose #3
HepB surface antibody (anti-HBs) ≥ 10 mIU/mL - immune
Anti-HBs < 10 mIU/mL – revaccinate (3 doses) and retest anti-HBs
Still negative – nonresponder, need HBIG after exposure
Previously vaccinated but not tested? Test for anti-HBs
after an exposure; if negative, treat as susceptible.
Transmitted primarily through contact with
infected blood
Many people asymptomatic (symptoms
similar to HepB)
~1.8 % of Americans have been infected
with HepC, 3.2 million chronically infected
~ 12,000 deaths/year
Leading indication for liver transplant in U.S.
Percutaneous injury, esp. with deep punctures or extensive
blood exposures
~2% develop infection
Mucosal/nonintact skin exposures rarely documented
Proper cleaning/disinfection of surfaces important
HCV in dried blood samples remains infective for at least 16 hours
(Kamili et al., Infect Control Hosp Epidemiol 2007; 28:519-524)
Universal Precautions for Prevention!
NO VACCINE
Antivirals (interferon/ribavirin) can have serious side effects,
treatment lasts 24-48 weeks
Transmitted through contact
with infected blood/OPIM
1° infection
Asymptomatic phase
Symptomatic phase
transient, non-specific illness
(fever, malaise, muscle pain,
sore throat)
↑ susceptibility to opportunistic
infections, nonspecific
constitutional symptoms (night
sweats, weight loss, anorexia,
fever)
Advanced (AIDS)
one or more opportunistic
infections, CD4<200 cells/µl
> 1 million living with HIV/AIDS
~56,000 new infections/year
~20% don’t know they are infected
Florida ranks 3rd among states
in the number of reported
HIV/AIDS cases
Risk for HIV transmission after:
Percutaneous injury – 0.3%
Mucous membrane exposure – 0.09%
Nonintact skin exposure – low risk (< 0.09%)
57 documented occupational
infections in U.S. (139 possible infections)
84% resulted from percutaneous exposure!
No cure
No vaccine
Antiretroviral therapy – cocktail of 3 or more drugs,
costly, side effects, drug resistance
Always use Universal Precautions!
Risks of becoming infected after a needle stick injury:
35%
30%
30%
25%
20%
15%
10%
5%
2%
0.3%
0%
HepB
*If unvaccinated*
HepC
HIV
Engineering (safety equipment)
◦ Safety needles, sharps box, biosafety cabinet
Work Practices
◦ Cleaning work surfaces, not recapping needles
Personal Protective Equipment (PPE)
◦ Gloves, lab coat, face shield
Maximum protection when
these controls overlap
Sharps container
Biosafety cabinet
Cleanable work surfaces/chairs
Leak-proof transport containers
Safety needles/syringes
List of safety sharps devices available can be found at:
http://www.healthsystem.virginia.edu/internet/epinet/safetydevice.cfm#1
Know what they are and follow them!
Minimize splashes
Don’t recap needles
Know how to handle spills
Wash your hands!
No eating/drinking in areas where blood/OPIM
is handled or stored
NO!!
NO!!
Discard needles directly into sharps
container
Do not overfill the sharps box – close and
replace when ¾ full
Never attempt to re-open a closed sharps box
Circumstances Associated with Hollow-Bore Needle Injuries
NaSH June 1995—December 2003 (n=10,239)
35% disposal
related
FRESHLY DILUTED (w/in 24 hrs) 1:10 solution of
household bleach
EPA listed tuberculocidal disinfectant
◦ http://www.epa.gov/oppad001/chemregindex.htm
◦ Clorox, amphyl, lysol, sporicidin
Ethanol evaporates too quickly to be an effective
disinfectant!
Notify people in the area
2.
Don appropriate PPE (gloves, safety glasses)
3.
Place absorbent material on spill
4.
Apply appropriate disinfectant – allow sufficient contact time (30 min)
5.
Pick up material (watch for glass – use tongs or dust pan); dispose of
as biowaste
6.
Reapply disinfectant and wipe
For large/problematic spills, call EH&S Biosafety Office (392-1591)
1.
Container of undiluted household bleach
Several pairs of gloves
Safety glasses
Absorbent material
Biohazardous waste (autoclave) bags
Dust pan & scoop or tongs for broken glass
Place in a labeled bag or bucket and keep in areas
where biohazards are used
Pay attention to frequently missed
areas – fingertips, between fingers,
under jewelry
Wash hands after removing gloves & before leaving the work area
If no sink nearby, use hand sanitizer and then wash with soap and water ASAP
Wear it WHEN and WHERE you are supposed to
PPE should never be worn in common areas (offices,
hallways, bathrooms, cafeterias, etc) or when handling
common-use items (doorknobs, elevator buttons,
telephones)
It is also common courtesy – others don’t know what you
may have touched/where you have been
PPE must be supplied by the employer
It must fit, be suitable to the task (use common sense),
and cleaned or disposed of properly (this does not mean
taking it home to wash!)
◦ Gloves
◦ Face and Eye Protection
Surgical mask, goggles, glasses w/side shield, face shield
◦ Body
Gowns, aprons, lab coats, shoe covers
Absolutely no open toed
shoes in the lab!
Gloves
Never re-use or wash gloves!
Some chemicals may breakdown the glove –
use glove compatibility chart
http://www.ehs.ufl.edu/Lab/CHP/gloves.htm
Pay attention to how you remove your gloves!
WASH HANDS!
Site-specific!
Equipment, practices, and PPE used AT YOUR SITE to
protect you and others
Written down, reviewed, accessible, updated annually or
as needed
Template for SOPs:
http://www.ehs.ufl.edu/Bio/BBP/BBPSOPS.pdf
More stringent control measures
Work must be registered with
EH&S Biosafety Office (rDNA or
BA registration)
Enrollment in medical
surveillance program
Follow CDC/NIH BSL-2
containment practices at a
minimum
Wash wound with soap & water for 5 minutes; flush mucous
membranes for 15 minutes
Seek immediate medical attention (1-2 hrs max)
◦ In Gainesville, call 1-866-477-6824 (Needle Stick Hotline)
◦ In Jacksonville, 7am-4pm, go to Employee Health Suite 505 in Tower
1; Other hours, go to ER
◦ Other areas, go to the nearest medical facility
Notify supervisor
Contact UF Worker’s Compensation Office, 352-392-4940
Allow medical to follow-up with appropriate testing & required
written opinion
Type/amount of
fluid/tissue
Infectious status
of source
Susceptibility of
exposed person
Percutaneous
injury (depth,
extent, device)
Blood
Presence of HepB
surface antigen
(HBsAg) and HepB
e antigen (HBeAg)
HepB vaccine and
vaccine response
status
Mucous membrane
exposure
Fluids containing
blood
Presence of HepC
antibody
Immune status
Type of exposure
Non-intact skin
exposure
Presence of HIV
antibody
Bites resulting in
blood exposure to
either person
CDC PEP Guidelines:
http://www.cdc.gov/mmwr/PDF/rr/rr5409.pdf
http://www.cdc.gov/mmwr/PDF/rr/rr5011.pdf
Training records:
◦ Retain a minimum of 3 years
Medical records for immunization or post-exposure
follow up:
◦ Retain for duration of employment + 30 yrs (includes HepB
vaccination records, vaccination declination statement)
Confidential sharps injury log (type of device involved,
where and how injury occurred):
◦ Retain for 5 years from date of exposure
Warning labels must be placed on:
◦ Containers of regulated waste
◦ Refrigerators & freezers containing blood or OPIM
◦ Containers used to store, transport, or ship blood or
OPIM
Use red bags for waste containers
Biological Safety Office
Environmental Health & Safety
352-392-1591
www.ehs.ufl.edu
[email protected]
State regulations require that all employees who may have
contact with BMW receive:
◦ initial training at time of hire
◦ annual refresher training
Training shall cover:
Training must be facility and site specific
◦ Identification, handling, use of protective clothing, segregation,
storage, labeling, transport, procedures for decontaminating BMW
spills, contingency plan for emergency transport, and procedure for
containment and treatment of BMW.
◦ Training Log/roster must be kept for a minimum of 3 years
◦ Documentation of individual employee training is to be kept in their
department.
Any solid or liquid waste which may present a threat of
infection to humans
◦ Includes but is not limited to:
Non-liquid human tissue and body parts
Blood, blood products and OPIM (as defined in OSHA BBP standard) from
humans and other primates
Laboratory/clinical waste containing/contaminated with blood, tissue, cell
cultures & other potentially infectious body fluids
Laboratory/veterinary wastes containing human disease-causing agents
Discarded sharps (medical items intended to cut or puncture skin,
e.g. needles, syringe/needle combinations, lancets, scalpels)
Biomedical waste – specifically regulated by the State of
Florida Dept. of Health
◦ BMW is infectious for humans & a subset of biological waste
Various regulations affect biological waste (NIH, USDA, etc)
◦ Biological waste includes rDNA, animal, & plant pathogens
Hazardous waste is a non-specific term. At UF, most often
used to describe chemical waste or chemicallycontaminated waste (pharmaceutical waste also handled
as chemical waste).
Used, absorbent materials saturated with blood, body fluids, or
secretions/excretions contaminated with blood & absorbent
materials saturated with blood or blood products that have dried
(bandages, gauze, sponges, wound care material).
Non-absorbent disposable devices (flexible tubing, disposable gloves,
intact glass and hard plastic, etc) contaminated with blood, body fluids,
or secretions/excretions contaminated with blood but have not
been sterilized or disinfected by an approved method.
Other contaminated solid waste which represents a significant risk
of infection b/c they are generated in medical facilities which care
for persons suffering from diseases requiring isolation criteria.
Described in Chapter 64E-16 FL Administrative Code
http://www.doh.state.fl.us/environment/community/biomedical/pdfs/64E16_1.pdf
Segregation, handling, labeling, storage, transport &
treatment are regulated. Prescribe specific:
◦ Sanitary practices
◦ Training
◦ Biomedical waste plan – provides guidance & describes
requirements for proper management of biomedical waste at the
generating site or facility
Permits req’d to generate, store, treat, & transport BMW
Inspections by the state
Enforcement
Content of this presentation closely follows the UF
Biomedical Waste Plan which is located in EH&S Biosafety
Office. Contact 352-392-1591.
The BMW plan for Shands UF is located in the
Environmental Services Dept., Room G137 (South Tower)
and Room B301.8 (North Tower). Contact 352-265-0480.
Other departments at UF/Shands may create and maintain
a BMW plan for their area using the following template:
http://www.doh.state.fl.us/environment/community/biomedical/index.html
International biological hazard symbol on the
container
The phrase “Biomedical Waste”, “Infectious
Waste” or “Biohazardous” must be on the
container
Bagged waste must be in red bags.
Segregated at point of origin into its proper container
◦ “Point of origin” is the lab, patient/exam/procedure room or other
area where the BMW is generated
Choices for proper BMW container:
◦ Red biowaste bag
◦ Labeled fiberboard box lined with a biowaste bag
◦ Sharps container – puncture resistant container specifically
designed for sharps
Chemically or radiologically contaminated gloves, tubes,
etc. do not go into a biomedical waste box. They go into
their appropriate waste container.
Call EH&S (352-392-1591) before putting hazardous
(chemical) or radioactive warning stickers on biomedical
waste containers.
Remember – the biomedical waste box is not a universal
disposal container!
Never Re-Cap Needles or Scalpels
◦ Don’t bend, break, or detach from syringe
Discard directly into a leak-proof,
puncture resistant container
Replace container when ¾ full
Label container with facility’s name and
address prior to offsite transport
◦ UF labs - date, PI name, room and phone #
Container should be located where the sharps are
used: patient rooms, procedure areas, exam rooms, lab,
etc.
◦ UF uses disposable containers transported for disposal by
Stericycle
◦ Shands uses reusable containers which are supplied & serviced
by Bio Systems
Only sharps should go into sharps containers
◦ Soft items quickly overfill containers and may cause sharps to
stick out of the top of the box. Sharps boxes containing items
other than sharps and syringes need to be replaced ASAP, but
definitely within the 30 days of first use
RED autoclave bags – must meet certain documented standards
of State of FL, BBP & DOT
◦ e.g. Fisher #01-828E (Medical Action Industries)
Red bags are to be available where needed
No liquid waste in red bags!
Once sealed, containers must stay sealed. If container breaks or
is punctured, put the whole broken container in a new one.
◦ Infectious/potentially infectious waste must be stored in a
covered, leak-proof container
BMW must be staged in an area away from general traffic & accessible
only to authorized personnel. Storage area must be:
◦ Labeled with biohazard sticker
◦ Secure (locked/non-accessible)
◦ Easily cleanable & tidy
Waste cannot be stored > 30 days
◦ “The 30 day period shall commence when the first non-sharps item of biomedical
waste is placed into a red bag or sharps container, or when a sharps container
containing only sharps is sealed.”
Packages must be labeled as biomedical waste with the biohazard
symbol, lab name, location, phone & date
Some locations stage the waste & then transport it to outdoor containers
removed for disposal by a designated hauler
Staging locations provided on each floor/as needed
◦ In some areas, BMW is picked up from the “point of use” location
North & South Tower staging: the Soiled Holding Room
on each nursing unit
South Tower: Rm 1172 ED Soiled Hold, Rm 1185 ED Soiled
Hold, Rm 1236 Radiology Soiled Hold, Rm 8219 Soiled Hold, Rm
7121 Soiled Hold, Rm 2136 OR Soiled Hold, G316 Biowaste
Staging, etc.
North Tower: Soiled holding areas on patient care and treatment
areas, CSS and OR soiled holding area such as 3236, 2426,
3522, 6445, 9515 or 11-532, etc
Wear appropriate PPE (gloves, clothing cover, safety
glasses) when handling non-inactivated waste
Use Universal Precautions – assume all BMW is
infectious
Transport waste in leak-proof containers
Know how to handle spills
At UF:
◦ Labs must furnish their own PPE and red bags (Fisher #01-828E)
◦ Sharps containers and BMW boxes are available from Building
Services custodians or from AG133 at the Health Science Center
(call 392-5775) or from Physical Plant Stores, Bldg 705 near the
Motor Pool (call 392-1115)
At Shands, staff may obtain any of the supplies by:
◦ Contacting Environmental Services at 352-265-0480 or
◦ Speaking to an Environmental Services staff associate on the unit
they are working
Transportation of BMW is provided by the following
registered BMW transporter:
Stericycle, Inc.
4245 Maine Ave
Eaton Park, FL 33840
407-361-5454
State of Florida Permit # 53-64-00911
Stericycle has a number of other sites in the state that
they can pull transport equipment from to facilitate
emergency situations
BMW shall be treated by heat, incineration, or other
equivalent method suitable for hazard inactivation
acceptable to the State of Florida.
Shands/UF BMW is treated by Stericycle, Inc.
◦ Autoclave which sterilizes the waste or
◦ Incineration which destroys the waste
Note: CJD BMW must be marked for incineration per hospital
policy by the area that has filled the container. See ICP 03-15,
Guideline for the Management of Patients with Suspected or
Confirmed Creutzfeldt-Jakob Disease (CJD) or other prion
disease
At UF, all lab waste handled by UF custodial
staff
UF Policy:
◦ Laboratory waste containing infectious, potentially
infectious, or rDNA organisms must be inactivated prior to
disposal
◦ Properly performed autoclave or bleach treatment is
acceptable
◦ Storage of all non-inactivated waste in this category is
restricted to within the generating laboratory
◦ Specific requirements apply for waste containing biological
toxins. Contact the Biological Safety Office at 352-3921591
Requirements:
◦ Biological indicator testing every 40
hrs of use (every 6 mos if autoclaving
non-infectious material exclusively)
◦ Log book
◦ Regular maintenance
◦ 250°F/121°C, 15-20 lb pressure
Large loads/resistant pathogens
need more time
◦ Typical bag of Biohazardous Waste =
60 min
Transport BMW to autoclave in
closed bag and leak-proof
container
Acceptable for liquid material if done correctly
◦ Add full strength household bleach to final concentration of
10% (5000 ppm available chlorine).
◦ Mix. Contact time should be at least 30 minutes.
◦ Pour down drain to sanitary sewer.
Beware of other disinfectants = “hazardous chemicals”,
harmful work with and can’t go down drain, must be
picked up by EH&S
Proper spill handling:
◦
◦
◦
◦
◦
Notify people in the area
Don appropriate PPE
Place absorbent material on spill
Apply appropriate disinfectant – allow sufficient contact time (30 min)
Pick up material (watch for glass – use tongs or dust pan); dispose of
material into biomedical waste
◦ Reapply disinfectant and wipe
◦ For large/high hazard spills, call the Biosafety Office (352-392-1591)
For routine disinfection of surfaces where BMW is handled,
use a 1:10 solution of freshly diluted bleach or a
tuberculocidal disinfectant (ethanol evaporates too quickly!)
◦ Shands uses VIREX, a hospital level disinfectant/germicidal cleaner
(Didecyl dimethyl ammonium chloride) to clean and disinfect surfaces.
Contact: The UF Biological Safety Office
352-392-1591
[email protected]
www.ehs.ufl.edu