Transcript My Favorite Pathway

My Favorite Pathway:
Glycolysis/Gluconeogenesis
Pallavi Penumetcha
11/25/08
Glycolysis (RAST):
Glycolysis Pathway Condensed:
Example of How to Investigate One Enzyme
Enzyme 3.1.3.11 was not highlighted in the
CDD Results
SEED pathway
Pfam Results
NCBI Results
Enzymes not called by RAST annotation
EC Number and Name
Other Databases?
Notes
3.1.3.11 (fructose
bisphosphatase)
Yes: SEED
2.7.1.11 (6phosphofructokinase)
Yes: SEED and Manatee
•5 calls between 2
databases (2 in SEED and 3
in Manatee)
•2 sequences in SEED match
with two in Manateecall
different EC numbers
-SEED: 2.7.1.11
-Manatee: 2.7.1.56
(1-phosphofructokinase)
•1 sequence different in
Manatee
•2 sequence match: same
results in CDD
•Sequence in Manatee has
the superfamily related to
phosphofructokinases
1.2.1.12 (glyceraldehyde3-phosphate dehydrogenase,
phosphorylating)
Yes: All three
•All three have same
protein sequence
•SEED calls the EC number
1.2.1.59 (glyceraldehyde3-phosphate dehydrogenase
NADP+, phosphorylating)
•Shows that the different
databases have different
criteria for calling a
protein
•Confirmed as fructose
bisphosphotase in CDD,
Pfam and NCBI
Fructose and Mannose Metabolism:
Pentose Phosphate Pathway