Transcript Ch 16 DNA Replication, Baby!

DNA
Replication –
Exciting
stuff from
chapter 10!
Ch 10 DNA Replication, Baby!
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DNA workshop – interactive basic overview:
http://www.pbs.org/wgbh/aso/tryit/dna/shockwave.html
Link to replication animation w/ no sound:
http://www.johnkyrk.com/DNAreplication.html
Link to genetically engineered corn video:
http://www.youtube.com/watch?v=n9xpKx03g9M&feature=player_embedded
Link to narrated DNA replication
http://highered.mcgrawhill.com/sites/0072437316/student_view0/chapter14/animations.html#
Link to DNA replication
http://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht
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Review Structure
The "rungs" are the nitrogenous base pairs
a. complementary base pairing
Uniform diameter
Review DNA function
• Stores information
• Mutates
Replicates
Expressed
DNA REPLICATION
When and where?
How about in bacteria?
Bacteria replication
of DNA:
one origin of
replication
DNA replication in eukaryotes:
Contain hundreds of origins and
complexes make replication
“________” that eventually fuse
Replication is called______ _________, because one
half of the original strand is always saved, or
"conserved"
not
Process of DNA replication:
1. enz. helicase unwinds and breaks H-bonds
2. single-stranded binding proteins stabilize
the molecule
3. enz. _________ adds a small piece of _____ (called a
primer) to origin of replication (DNA nucleotides
can only be added to the end of an _________ chain)
4. enz. DNA ____________adds new dNTPs to the ____
end - A-T, G-C, 5'3 direction of growing strand
leading strand - one primer needed
lagging strand - Okazaki fragments primer needed for each fragment
5. A dif. DNA polymerase replaces RNA
___________ w/ DNA
6. enz. DNA ________ joins Okasaki fragments together
Ch 10 DNA Replication, Baby!
•
•
•
•
•
•
•
•
•
•
DNA workshop – interactive basic overview:
http://www.pbs.org/wgbh/aso/tryit/dna/shockwave.html
Link to replication animation w/ no sound:
http://www.johnkyrk.com/DNAreplication.html
Link to genetically engineered corn video:
http://www.youtube.com/watch?v=n9xpKx03g9M&feature=player_embedded
Link to narrated DNA replication
http://highered.mcgrawhill.com/sites/0072437316/student_view0/chapter14/animations.html#
Link to DNA replication
http://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht
m
Telomeres - repetitive
sequences of nucleotides at
the ends of DNA
• shorten w/ each rep.
• purpose?
Enz. ______________extends telomeres in certain cells
1. stem cells
2. cancer cells
Can mistakes that occur to DNA be repaired?
Excision repair - repair of
damage due to chemicals,
radiation, X-rays, UV light
What is the result of
uncorrected errors?