Transcript Streptococcus pyogenes Staphylococcus aureus Escherichia coli

Euroimmun/Lund University
Meeting
Heiko Herwald
Lund University
Sweden
Lübeck July 7, 2016
Sepsis is a complex syndrome
Streptococcus pyogenes
Staphylococcus aureus
• Infecting pathogen
• Site of infectionFigure 2:Malmström et al
a)
• Disease spectrum
Escherichia coli
Salmonella spp.
“The microorganisms ... turn out ... to be rather more
like bystanders. It is our response to their presence
that makes the disease. Our arsenals for fighting off
bacteria are so powerful ... that we are more in danger
from them than the invaders.”
Lewis Thomas (1972)
Host parasite interactions
Streptococcus pyogenes
Staphylococcus aureus
Escherichia coli
Salmonella spp.
Pathogenesis of Severe Sepsis
Infection
Microbial Products
(exotoxin/endotoxin)
Cellular Responses
Platelet
Activation
Coagulation
Activation
Oxidases
Kinins
Complement
Coagulopathy/ Disseminated Intravascular Coagulation
Vascular/Organ System Injury
Multi-Organ Failure
Death
Cytokines
TNF-, IL-1, IL-6
cell culture
RT-PCR
in vivo
patient material
sample processing
mass
spectroscopy
BiaCore
Bio-Plex
IVIS
electron
microscopy
microscopy
QuantStudio™ 7 Flex Real-Time PCR System
RT-PCR
• Real-Time PCR System
• broad range of real-time PCR-based applications
through
• multiplexing capabilities
• interchangeable block formats. With a simplified
• exceptional reproducibility with minimal well-to-well
and instrument-to-instrument variation.
SPR biosensor BiaCore T200
BiaCore
•
•
•
•
label-free system for detailed studies of biomolecular interactions
measurements in real-time
analyze interactants ranging from ions to viruses
small concentrations needed
Bio-Plex 200 System
Bio-Plex
• suspension array system
• automates the reading of 96-well plates
• reliable multiplex assay solution that permits analysis of up to 100
biomolecules in a single sample
• yielding up to 9,600 data points in about 35 min
In Vivo Imaging Instruments PerkinElmer
IVIS
• non-invasive real-time measurements in living animals
• 2D and 3D quantitative bioluminescence and fluorescence with
fast and low dose CT imaging
• the system is capable of both luminescence and fluorescence
modes of imaging
Electron microscopy
electron
microscopy
• high resolution
• morphological studies
• structural studies
Microscopy
microscopy
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•
•
•
tissue morphology
histology
immuno-histology
co-localization
cell culture
RT-PCR
in vivo
patient material
sample processing
mass
spectroscopy
BiaCore
Bio-Plex
IVIS
electron
microscopy
microscopy