Seed Inoculums - Amazon Web Services
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Seed Inoculums &
Activators
BioNutrient Food Association
Soil and Nutrition Conference 2016
Crop Services International
Your partner in Successful Conversion to Sustainable,
Biological, Organic and Energetic for over 40 years
Dane Terrill
Soil Testing and Interpretation
Consulting
Remineralization
Biological Activation
Soil Foodweb Advisor
Fertility Recommendations
Supplier of Quality Products
www.cropservicesintl.com
Compost Tea Spray Service
Organic Lawn Care
Biological Growing Consultant
Biological Testing and Interpretation
Educational Talks, Lectures and Walks
7700 Sprinkle Rd, Portage, MI 49002
616-246-7933
Vermicomposting
Soil Biology Crash Course
Very very large number of microbes in HEALTHY soil? How Large
Perspective
Counting - 1 number per second
Million ≈ 12 days
Billion ≈ 31 years (6 Billion Microbes in healthy soil)
Trillion ≈ 32 thousand years
Is our current understanding of the number of microbes correlated to
the time to count them?.
© Crop Services Intl
www.cropservicesintl.com
Source: Soil Biology Primer 2000
Biological Components of a Healthy Soil
• Not from a Stethoscope
• From a Microscope “Let’s take a look”
© Flowerfield Enterprises, LLC
Bacteria
Decomposers, Solubilizers, Mutualists,
Rhizobia, Azotobacter, B. subtillus and B. thuringiensis (Bt)
Alkaline by nature
Physical Shapes
Round-cocci
Rod- Bacillus
Spiral- Spirochete
Notes
Largest % of N
Virile
Out of balance in soils
Compaction
Weeds
Bacterial diseases
Nutrient Leaching
Bacteria
Compost Tea
400 X Mag
© Flowerfield Enterprises, LLC
Fungi
Decomposers- break down Lignin and Cellulose
Mutualists- symbiotic relationship with plants (Mycorrhizae)
Pathogens- to plants (causing loss) to microbes (nematodes)
Acidic by nature
Common: Trichoderma, Mycorrhizae, Verticillium, Clonostachys,
Beauvaria
Physical Shapes
Hyphae-thread like structures
Notes
Convert OM into Humus (Sequester carbon)
Out of balance in soils
Compaction
Nutrient Leaching
Lack of fungal disease suppression
Fungi
Mycorrhizal Fungi
© Flowerfield Enterprises, LLC
Protozoa
Nutrient Cycler in the soil (N)
Common: Ciliates, Flagellates, Amobae
Consume Bacteria and other protozoa
Create soil aggregates
Protozoa Math
8000 N molecules mineralized/protozoa/day
X 50000 protozoa/gram of healthy soil
400,000,000 N molecules made available
=
7ng of N/gram of soil/day
Growing plants need 0.2ng of N/gram/day
Flagellate
Ciliates
Protozoa
Rotifer
Amoebae
Nematodes
Non-segmented worm-like microbe
Most are beneficial
Nutrient Cyclers, eat bacteria, fungi and nematodes
Common
Bacterial Feeders
Fungal Feeders
Switchers
Parasitic
Vermicompost typically is loaded with Nematodes
Fungal-feeder
Nematodes
Bacterial-Feeder
Bacterial-feeder
Predatory Nematode
Root Feeder
Ever seen these?
Hint: not a GMO carrot
Here is the culprit
Microbes function in Healthy Soil
Residue Management:
They decompose the plant residues.
Seed Germination:
Protect first radical from soil pathogens
Solubilize minerals
Growth/Flower/Fruiting Stage:
Liberate the Nitrogen and Minerals necessary for plant
growth
Protect roots, shoots & fruits from bacterial, fungal and
insect outbreaks
Produce organic plant foods from organic residues in the
soil
Build and improve soil structure
Balance
Seed Germination
Seed swells up with moisture, cracks and first radical
shoots out and down
Like the intestinal tract of a newborn child void of any gut
flora, the radical is vulnerable
Critical time for inoculation of beneficial microbes
(bacteria, fungi, protozoas and nematodes)
Inoculation & Protection
Needs of the Radical
Radical’s job is to anchor the plant (main root)
Scavenging for soluble nutrients particularly (P)
Nutrient Solubilizers are critical at this stage
(Consortiums-Not individuals)
Carbon as a food source
Moisture
Minerals and Trace Minerals
Looking for air spaces in the soil for root to penetrate
Nutrient Solubilizers
Biological Seed Inoculums
No seed treatment
Seed Treatment ½#/Acre
Applied with 2 g of fish, 1 pt. of Kelp, and 1 g molasses.
Specific Microbes and Families
Acinetobacter – colonize seedling within 3 days, solubilizes P, eats diesel oil
Azotobacter – bacteria, converts soil N and atmospheric N in soil and on foliage
Bacillus- bacteria, secretes plant growth hormones and antifungal compounds
Pseudomonas- bacterial solubilizers, breaks down toxins
Peptococcus activus- enhances germination in high cadmium soils(rhizosphere)
Pencillium – fungi that solubilizes P and Ca (soil)
Trichoderma- antagonistic fungi against pathogens (soil)
Clonostachys – antagonistic fungi against pathogen, colonizes root (rhizosphere)
Mycorrhizal- symbiotic with plant roots, nutrient solubilizer (rhizosphere)
2016 over 2000 individual species tested on 500,000 acres
Consortiums
Consortiums
Adding multiple species together creates symbiosis
T. harzianum with B. subtillus
Rebuild or mimic the Soil Foodweb
Compost, vermicompost
Biological Products
We simply don’t have enough scientific data (2-5%) to use
isolates alone.
Diversity
Diversity
Nitrifying (Azotobacter, Bacillus,
Pseudomonas)
Solubilizing (Pseudomonas, Bacillus,
Mycorrhizal fungi, Protozoas)
Decomposing (Bacteria, Fungi)
Insecticidal (B. thuringienis, B. bassiana,
V. leccani)
Fungicidal (Trichoderma, Clonostachys)
Make your own Inoculum
• Compost and/or Teas/Extracts
– Use indigenous species
– Add diversity, soil from woods, compost, worm castings
– Add Kelp or Seawater
– Add Carbon (humates, biochar (loaded)
– Spray on, put in furrow, or soak seeds
Good
Results
Soybeans
Planted May 5, 2008
5 gallons CT/acre
in furrow
20 gallons CT/acre
soil drench w/humic
5 gallons CT/acre
Foliar feed (6/01/08)
5 gal. CT/acre
Foliar feed (7/4/08)
Soybean root: 22 inches long- 6/19/08
5 gal. CT/acre
Foliar feed (8/16/08)
Note: compaction layers at 11” and 18”
Yield: 41.6
Bushel/Acre
Cass County Ave: 36
bushel/acre
Broad Spectrum Inoculums
Diversity– Worm Castings/compost
Groups Added
Nitrifying
Solubilizers (mineral and trace minerals)
Protectors (bacteria and Antagonistic fungi)
Kelp – Plant Growth Hormones Cytokinin's, Auxins and Gibberellins
Soluble (immediately available) and Raw(slow release)
Trace minerals
Mycorrhizal – good balance of biological activity for colonization to
occur. (weak sister)
Carbon – food source for the microbes until exudates start to flow
Better
Specific Inoculums
Use good or better inoculum
Add known species for specific crops or conditions
Trichoderma family- antagonistic
Bacillus subtillus- anti fungal compounds
Bacillus thuringenis- insecticidal properties
Clonostachys rosea- fungal pathogen suppression
BEST
Adding Specific Microbes
Keys to Seed Inoculums
Balance
Bacteria, fungi, protozoa, nematodes
Diversity
If we only know 2-5%, why target individual species
Protectors
Consortiums are better than individual
Solubilizers
Nutrition is critical for genetic potential (even in cold soils)
Activators – Carbon (fish, molasses, humic and amino acids)
Let Mother Nature sort it out work
Questions, Comment or Concerns
Dane Terrill
Crop Services International
7700 S Sprinkle Rd
Portage, MI 49002
269-757-3519 cell
800-260-7933 office
[email protected]
www.cropservicesintl.com