Transcript Microscopes

Microscopes
Instruments used to magnify &
view objects
In the beginning…
Diseases were thought to be caused by
“supernatural spirits” and “curses”
Anton van Leeuwenhoek
●1600’s
●1st view of “animalcules”
oAKA cells & bacteria
●Simple Light Microscope, only 1 lens
Robert Hooke
●English scientist
●Compound light microscope
o2 or more lenses
●Looked at cork (dead cells on oak bark)
oSaw small geometric shapes, named them
CELLS
●Cells-> are the basic units of all living
things
●http://www.wwnorton.com/nael/18century/topic_3/microscope.htm
What did he see?
http://www.cbs.dtu.dk/staff/dave/roanoke/bio101ch05.htm
Then What?
●1830’s
●More scientists used Hooke’s ideas to
explore the natural world
●Matthias Shleiden, a german
oPlants are composed of cells
●Theodor Schwann
oAnimals are composed of cells
●Summarized in the Cell Theory
Cell Theory
The Cell Theory:
3 Main Ideas:
1.All organisms are composed of 1 or
more cells.
2.The cell is the basic unit of structure
& organization of organisms.
3.All cells come from preexisting cells.
Types of Microscopes
Types of Microscopes
●Light Microscope: what we use in lab
oUses light to magnify
oUsed to look at living cells
oMagnify: up to 1500x
●Electron Microscope: (1930-1940)
oUses beams of electrons
oMagnify: up to 500,000x
oSee inside of cells, look at structures
o2 types
Scanning Electron Microscope:
(SEM)
●Scan surfaces of cells, look at 3D shape
http://www.mos.org/sln/sem/diatom.html
http://mse.iastate.edu/microscopy/whatsem.html
Transmission Electron Microscope
(TEM)
●Look at structures within the cell
http://en.wikipedia.org/wiki/Transmission_electron_microscope
New Technology
●New models & helpful techniques all the
time
●Example: Scanning Tunneling Microscope
oUses flow of electrons to create computer
images of atoms on the surface of a molecule
www.chem.utoronto.ca/staff/DHIRANI/index.htm
The Cell:
●With better microscopes, discovered that
cells contain small specialized
structures called organelles
●Each organelle has a specific function
within a cell
Types of Cells:
● 2 broad groups:
1.Eukaryotes: contain membrane-bounded organelles (ex.
nucleus)
 Most multi-cellular organisms & some unicellular ones, like
algae
 Complex cells
2.Prokaryotes: do not contain membrane-bound organelles (ex.
No nucleus, but nucleoid region when chromatin is stored).
 Example: bacteria
 Less complex
 Many functions occur on cell membrane
What do they look like?
http://en.wikipedia.org/wiki/Eukaryotic
http://www.earthlife.net/prokaryotes/im
ages/bacteria.gif