Transcript Photosynthesis Lab Review - Kirchner-WHS

Photosynthesis Lab Review
Photosynthesis
• Plants, algae, and some bacteria convert light
energy into chemical energy that is stored in
sugars
• Chlorophyll is the primary
pigment in chloroplasts
Plant Pigment Chromatography
• Paper chromotography is
used to separate a
mixture into its
component molecules
• The different molecules
move up the paper at
different rates because
of differences in:
– Solubility, molecular
mass, and hydrogen
bonding with the paper
• You separated
plant pigments
using an
organic solvent
(acetone)
Analysis of Results
• Movement of
pigment relative to
movement of solvent
is a constant, Rf
(Reference front)
• Rf = distance pigment
moved/distance
solvent moved
Photosynthesis—Light Reactions
• Light energy excites electrons in plant pigments
• Boosts them
to a higher
energy state
• High energy
electrons
reduce
electron
acceptors in
the thylakoid
membrane
• Energy is
captured in
NADPH & ATP
DPIP as an Electron Acceptor
• You measured rate of electron excitation when
light hits chlorophyll
• DPIP is an electron acceptor
• As DPIP is reduced, it changes from blue to
colorless
• To measure the color change, you used a
spectrophotometer
– Measures amount of light absorbed or
transmitted by the sample
Design of the Experiment
Results
• As chlorophyll’s
electrons are
exited, they
reduce the
DPIP, and the
sample
becomes more
and more pale,
and the
transmittance
increases
Cellular Respiration Lab
•
•
•
•
Occurs in every cell of all living things
Mitochondria
Nutrients (sugars) convert ADP to ATP
ATP used for all cellular activities that require
energy
• C6H12O6 + 6O2 -------->
6CO2 + 6H2O + ~38 ATP
Design of the Experiment
• You measured amount of oxygen consumed
by peas using a respirometer
Design of the Experiment
Results
–  temp =  respiration
–  germination =  respiration