Transcript MultiChrome Culture Results: Gram Positive

MultiChrome™ Microbiology
Culture System
Sensi-Ring™ Antibiotic
Sensitivity System
Case Studies Reference Library
Kacey Diagnostics © 2010-14 All Rights Reserved
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Sensi-Ring™
Antibiotic Sensitivity System
Forward: This library is designed to help those using the Kacey Diagnostics
C&S System with real world cases in which its unique chromogenic media has
been used to identify bacteria and further discover its sensitivity to various
antibiotics. These images were taken directly from the field and have
not been retouched. This is a work in progress and are updating this library
almost daily so please visit us often.
We want to thank in particular Dr. Louis Gotthelf for his invaluable help in
compiling this library and to the many hospitals and clinics using this system
for sharing their findings with fellow colleagues.
Any use of these images in a manor not approved by Kacey Diagnostics
without its written consent is strictly prohibited.
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INDEX:
Type of Colony
Pages
Gram Negative
Gram Positive
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Ear Infections
5
UTI
6-7
Skin/Wound
Mixed Colonies
8-10
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Type: Gram Positive
GP
Patient/Breed: “Apollo”, 6 yr old Cocker
Culture Source: Recurrent Ear/Skin infections
MultiChrome Culture Results:
Gram Positive: Staph/Strep both ears
Sensitivity Results:
Notes: Because these are both GP organisms,
a GP Sensi-Ring was used. This is a very interesting
culture because the Staph is sensitive to many of the
common drugs including Penicillin. The Streptococci,
however are resistant to enrofloxicin, which is typical
for Streptococci. They do not usually respond to f
luoroquinolones as is demonstrated here.
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Type: Ear
GN
GP
Patient/Breed: “Jessie”,
Culture Source: R Ear
MultiChrome Culture Results:
Gram Negative: E. coli
Gram Positive: Enterococci
Sensitivity Results:
Notes: Mueller Hinton plate was swabbed
with both GP and GN material.
5
Type: UTI
GN
Patient/Breed: “Molly”, 19 yr old Feline
Culture Source: Urine
MultiChrome Culture Results:
Gram Negative: E-coli
Sensitivity Results:
Notes: A Mueller Hinton plate was inoculated with
the sediment as well. A Kacey UTI sensi ring was
placed over the growth and incubated for 24 hours.
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Type: UTI
GN
GP
Patient/Breed: “Peaches”, 10 yr old M/Feline
Culture Source: Urine
MultiChrome Culture Results:
Gram Negative: E-coli, Kleb
Gram Positive: Enterococci
Sensitivity Results:
Notes: Only the Augmentin disk (amoxicillin/clavulanate )
and the Trimethoprim/Sulfa disk showed sensitivity to all
bacteria. The Gentamycin (GM) disk showed sensitivity,
but the zone of inhibition was too low to be therapeutic.
.
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Type: Mixed Infection
GN
GP
Patient/Breed: “Baby”, Pomeranian
Culture Source: Anal Glands
MultiChrome Culture Results:
Gram Positive: Enterococci
Gram Negative: E-coli, Kleb
Sensitivity Results:
Notes: Antibiotic used “Ciprofloxacin
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Type: Mixed Infection
GN
GP
Patient/Breed: “Kizzy”, 3 yr old F Pug
Culture Source: Pododermatitis
MultiChrome Culture Results:
Gram Positive: Staph & Strep
Gram Negative: Pseudomonas &Kleb
Sensitivity Results:
Notes:K izzy has had demodex mange with
pododermatitis for at least one year. There was so much
swelling and exudation on the feet that the owner put socks
on the dog’s feet to prevent licking. This dog has been
treated for mange and bacterial skin infection by several
different vets with no effect. On presentation, the feet were
weeping purulent material. A culture was taken of the pus
that was squeezed out of the skin lesions after the feet
were disinfected in chlorhexidine.
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Type: Mixed Infection
GN
GP
Patient/Breed: “Zoe”, Maltese
Culture Source: Anal Glands
MultiChrome Culture Results:
Gram Positive: Listeria
Gram Negative: E.-coli
Sensitivity Results:
Notes: Antibiotic used “Clavamox”
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Kacey Diagnostics © 2010-14 All Rights Reserved
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