Transcript BactoCount IBC/IBCm - Bentley Instruments Inc

Innovative Analytical Solutions for the Dairy Industry
BactoCount IBC/IBCm
• Highly standardized method
• Rapid & accurate determination of
Milk Total Flora & Somatic Cells.
BactoCount
IBC
BactoCount
IBCm
Overview
• BactoCount Method’s General Principle
• The BactoCount Universal Conversion Equation
• Methods Standardization: a prerequisite (ISO Standards)
• Findings from a 10 year- 13 country- Study
• BactoCount International Proficiency Ring Test
• Conclusions
BactoCount Method’s General Principle
Flow Cytometry
Total Flora (10’)
Somatic Cells
1. Degradation of milk biological interfering
components by… chemical, heat and mechanical or
ultrasonic treatment
2. Destruction of the bacterial aggregates to count
Individual Bacteria (IBC) instead of Colony Forming
Units (CFU)
3. Staining of the Bacteria DNA/RNA
4. Detection of Individual Bacteria (IBC)
5. IBC translated to Colony Forming Units (CFU) with a
Conversion Equation (ISO 21187)
• Mixing of Milk & Buffer
• Staining of the somatic
cells DNA with
fluorescent marker
BactoCount Method Standardization
ISO 9001:2008 Production Certified
Company
Lyophilized Certified Bacteria/SCC
Standard
The BactoCount Universal Conversion Equation
• The Conversion Equation includes all relevant variations
 Bacteria types and growth phase
 Storage conditions
 Regional influences & production conditions
 Seasonal variations
 Sample preservations
For a robust Conversion Equation, you must use natural raw milk samples and
evenly cover the entire working range of 100 samples/Log unit.
BactoCount Universal Equation (2010)
BactoCount v. ISO 4833
11 Countries – 13 BactoCount – 8 Years – 4413 Samples
Countries
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Brazil
Czech republic
Estonia
France
Germany
Ireland
Italy
Lithuania
Switzerland
Turkey
USA
BactoCount Universal Equation (2011)
BactoCount v. ISO 4833
12 Countries – 15 BactoCount – 9 Years – 6504 Samples
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Brazil
Czech republic
Estonia
France
Germany
Ireland
Italy
Japan
Lithuania
Switzerland
Turkey
USA
BactoCount Universal Equation (2013)
BactoCount v. ISO 4833
14 Countries – 22 BactoCount – 11 Years – 9735 Samples
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Brazil
Czech republic
Estonia
France
Germany
Ireland
Italy
Japan
Lithuania
Poland
Switzerland
Turkey
USA
Finland
BactoCount European Conversion Equation (2013)
BactoCount v. ISO 4833
11 EU Countries – 22 BactoCount – 10 Years – 7706 Samples
BactoCount Universal Conversion Equation
Robustness
BactoCount IBC
ISO Accredited International Proficiency RT (CECALAIT)
Protocol:
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10 Preserved raw milk samples: 10-300 kCFU
2 IBC standard RM (lyophilized)
1 Microsphere Working Solution RM (MS)
Samples analyzed in duplicates
Results reported in IBC
BactoCount IBC
ISO Accredited International Proficiency Test
22 BactoCount – 14 Countries – 0% lab. Outside target
• Sr= 0,09 log
• SR= 0.072 << 0,16 log
• Sy,x= 0,035 log << 0,40 log
BactoCount fully complies with ISO
16140 requirements
BactoCount Reproducibility is within
the ISO 16140 Repeatability
requirement
BactoCount IBC Accuracy (Universal CE)
CECALAIT ISO 17043 International Proficiency Test
BactoCount IBC v. ISO 4833
BactoCount IBC
CECALAIT ISO 17043 International Proficiency Test
BactoCount Accuracy
BactoCount v. ISO 13366-1/IDF 148-1 (Somatic Cells)
BactoCount IBC/IBCm
Analytical Specifications
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Flow Cytometry
Powerful Laser
Very High Sensitivity
Long Integration Time
Outstanding Performances
BactoCount IBC/IBCm
Real-time detection of potential contaminations in raw milk
In the lab
• Rapid and highly accurate
determination of:
 Bacteria Contamination in raw
milk from cows, sheep, goats and
buffalos.
 Bacteria contamination in
individual animals, bulk tank, milk
tankers etc.
In the field
• Rapid control of the milk tankers
 To avoid silos contamination.
 Optimize milk segregation.
• Rapid control of the silos before
processing
 Adjusts pasteurization process
based on milk quality, detection of
contamination etc.
BactoCount IBC/IBCm
The BactoCount is the ultimate solution for rapid and accurate
calculations of the bacteria and somatic cells in milk
• Real time, Highly Accurate tests (TF, SCC...)
• Simple to use (lab operators, truck
drivers...)
• Dependable
• Versatile (open platform)
• Worldwide equivalence of analytical results
(TF/SCC)
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