Transcript sIgG - York College of Pennsylvania

Serum Immunoglobulin Production in Response
to Oral Bacteria in Canis familiaris
Lauren Brzezenski
Department of Biological Sciences, York College of Pennsylvania
Figure 2.
 Over 300 different bacterial species
live in the canine oral cavity (Elliott et
al. 2005).
sIgG Cells/g tartar (Mcpg)
Introduction
Methods
Canines
(n=10)
 Bacteria forms a biofilm on the teeth
which causes plaque and eventually
develops into tartar.
Collection of serum
(pre-anesthetic/
Pre-dental
blood draw)
 Gum recession caused by periodontal
disease allows bacteria to enter the
bloodstream & affect the liver, kidneys,
& heart.
 Immunoglobulin (IgG) production is
the major serum antibody response to
bacterial infections.
IFA
sIgG cell count
1000
 The p value is > 0.05 (Fig. 2) which implies
no significance (Spearman correlation analysis).
800
600
The p value is > 0.05 (Fig. 3) which implies no
significance (Spearman correlation analysis).
400
200
0
0
1
2
3
4
Stage of disease
Collection of tartar
during dental
procedures
Figure 2. Number of serum Immunoglobulin cells per gram
of tartar in relation to stage of periodontal disease in
canines.
DTAF
Total oral bacteria
Count from tartar
5000
Conclusions
There is no significant relationship
between the stage of periodontal
disease, the concentration of total
bacteria from tartar, & the concentration
of sIgG from tartar.
4000
3000
2000
1000
0
 The p value is < 0.05 (Fig. 4) which is
significant (Pearson correlation).
Canines produce sIgG against bacteria
found in tartar.
Figure 3.
Total cells/g tartar (Mcpg)
 The four major stages of periodontal
disease in canines are 1. Gingivitis, 2.
Mild, 3. Moderate, 4. Advanced. They
are distinguished by changes that occur
in the gums & teeth (Sims et al. 1990).
Results
1200
0
1
2
3
4
The concentration of sIgG specific for
bacteria in tartar increases as total
concentration of tartar increases.
Stage of disease
Figure 3. Total number of bacterial cells per gram of
tartar in relation to stage of periodontal disease
in canines.
Objectives
2. To determine if there is a
relationship between the stage of
periodontal disease in canines, the
concentration of bacteria in tartar
removed during cleaning, & the
number of sIgG specific for those
oral bacteria.
Elliott, D. R., Wilson, M., Buckley, Catherine M.F.,
and Spratt, D. A. 2005. Cultivable oral
microbiota of domestic dogs. Journal of Clinical
Microbiology 43, 11: 5470-5476.
Figure 4.
5000
sIgG
sIgG
Total cells/ g tartar (Mcpg)
1. To determine if canines produce
serum Immunoglobulin G (sIgG)
against bacteria found in tartar.
4500
4000
3500
3000
2500
2000
1500
1000
500
0
0
200
400
600
800
1000
1200
sIgG cells/ g tartar (Mcpg)
Figure 1. sIgG specific for oral bacteria.
Literature Cited
Figure 4. Serum Immunoglobulin cells per gram of
tartar in relation to total number of bacterial cells
per gram of tartar in canines.
Sims, T.J., Moncla, B.J., and Page, R.C. 1990.
Serum antibody response to oral gram negative
bacteria by cats with plasma cell gingivitispharyngitis. Journal of Dental Research 69: 877882.
Acknowledgements
Dr. Mathur for her constant help & support over the summer.
Fallston Veterinary Clinic doctors & staff for allowing me to collect
samples.