Transcript Cyanobacteria - Florence-Darlington Technical College

BIO 225 Microbiology


Ken Malachowsky
Florence-Darlington
Technical College
MICROSCOPE
Illuminates
Magnifies
Contrasts
SPECIMEN
Optics
Resolves
Distinguishes
Lens systems
Vary Light intensity
Condenser
OCULAR
OBJECTIVE
Lamp
Background
Distinguish
Ability to
Provide sharp, crisp
image
Total
magnification
Iris diaphragm
Multiply two powers
Two close
objects
Leewenhoek’s Simple Microscope
A Compound Brightfield Microscope
Scanning Power
X
4x
Low Power
X
X
4x
10x
High Power
X
X
4x
10x
4x
40x
40x
10x
100x
Field of Vision
Depth Perception
Steve Berg winona state university, used with
permission
Refraction
The use of Immersion Oil
Microscope Rules
• Never force anything on the microscope
• Inform the instructor if there is a problem with
the microscope
• Only use your assigned microscope
• Please do the following when you are finished
– Click the 4x objective in place
– Lower the stage far from the objective
– Place the microscope in its assigned space
Slides to View
• Letter E
• Colored Threads
• Cheek cells
•
•
•
•
Arthropod: Cyclops or Daphnia
Algae: Volvox, Spirogyra, or Stentor
Prototists: Amoeba, Paramecium or diatoms
Mixed Bacteria
• Pond water wetmount
KM
Cheek Cells
100x
400x
KM
Slides to View
• Letter E
• Colored Threads
• Cheek cells
•
•
•
•
Arthropod: Cyclops or Daphnia
Algae: Volvox, Spirogyra, or Stentor
Prototists: Amoeba, Paramecium or diatoms
Mixed Bacteria
• Pond water wetmount
KM
Cyclops
100x
KM
Daphnia
100x
KM
Volvox
100x
400x
KM
Spirogyra
400x
KM
Amoeba
100x
400x
KM
Paramecium
100x
400x
KM
Representative algae, or plantlike protists: The diatom
Campylodiscus hibernicus
These protists usually have cell walls surrounded by a loosely
attached test that contains silicon or calcium carbonate. Most
reproduce by binary fission. Some lack flagella.
Diatoms
400x
KM
Mixed Bacteria
1000x
Slides to View
• Letter E
• Colored Threads
• Cheek cells
•
•
•
•
Arthropod: Cyclops or Daphnia
Algae: Volvox, Spirogyra, or Stentor
Prototists: Amoeba, Paramecium or diatoms
Mixed Bacteria
• Pond water wet mount