Transcript lab practical review

Practical Review Practice( SemI)
Advanced Placement Biology
Laboratory Review Sheet
Dissolved oxygen simulation
http://www.phschool.com/science/biology_place/labbench/la
b12/varlight.html
Play the simulation.
Relate to an aquatic ecosystem
How does dissolved oxygen relate to primary productivity
How do the following variables affect dissolved oxygen
Temperature
Salt water and fresh water?
Running and still
Question and answers
If the water temperature increases, the dissolved
oxygen________
Decreases
In general running water has ________
( more or less) oxygen than still water
More
Nomogram
Read the % saturation
For a temperature of 25oC, what is
the % saturation ?
For a temperature of 25oC, what is
the dissolved oxygen?
Class Microscope.
http://www.microscope-microscope.org/basic/microscope-parts.htm
Identify the following
Objective lenses
Condenser
Mechanical stage contraol
Fine Adjustment
Coarse Adjustment
Rotating noewpiece
Be able to identify:the following
Oil
High
Low
Scanning
How to prepare a wet mount.
What are these steps?
What is this cell?
Starfish egg cell
Is this cell a eukaryote or a prokaryote?
What s the difference between these two
pictures of Spirogyra? How would Spirogyra be
classified?
What is
Volvox?
Why does this
show evolution?
Spectrophotometer
http://www.umd.umich.edu/casl/natsci/slc/spec21.swf - simulation
Spectrophotometer
What is absorbance?
What is percent transmittance?
What colors are associated with
the wavelengths of visible light
Chromatography with a Flair
Chromatography
What is the matrix?
What is the solute?
What is the solvent?
What is the basis of separation?
What is the Rf value?
Gel electropohoresis
What is an electrophoresis chamber ?
Describe how to put the sample
in the wells?
The sample is loaded at this end
of the gel box
This shows the molecules moving
toward the
DNA gel
What is the stain in the
picture?
What is the marker used
with this experiment?
DNA Electrophoresis
Which is the
marker?
Micromeasurement
What is the reading on this
micropipet?
Micropipet Use( review)
Review how to use the micropipet
http://www.fhcrc.org/education/hutchlab/lessons/use.html
What is an isolation streak?
What is flaming?
What is the shape of these bacteria? Which one is gram
positive?
What type of bacteria are these?
What type of bacteria are these?
What type of bacteria are these?
Kirby Bauer
What is resistance , what is sensitivity?