Transcript Curriculum Walk Through

What Is the
Amgen Biotech Experience?
http://www.bwbiotechprogram.com/
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Why should your students study biotechnology?
Why Biotech?
Amgen Biotech Experience
 Student and teacher curriculum
 Basic content, lab techniques, and skills used by
researchers in the biotechnology field
 Choice of lab sequences
 All specialized equipment provided including
consumables at no cost
 Teachers provide limited, commonplace equipment
(beakers, etc)
Basics of ABE
Problem: How can we mass produce a useful
protein?
Solution: Take the protein from the organism that
makes it and put it into something that will make
large quantities of that protein.
What are they doing?
Procedure
◦ Find the gene that makes the protein
◦ Cut the gene out
◦ Put the gene into a carrier
◦ Use the carrier to insert the gene into bacteria
◦ Turn on the gene so the bacteria makes the
protein
How do they do it?
Student Guide
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Diabetes and insulin production is used as an
example throughout the curriculum
Learning goals
Visuals
Literacy strategies
Relevant analysis questions
Glossaries
Teacher’s Guide
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Choice of lab sequences
Detailed teacher prep information with materials
lists, step-by-step instructions, and prep tips
Identified media resources specific to an activity
(on web-site)
Approximate timing is given for all activities
Supplemental activities support each lab
During the lab, specific questions encourage
student discourse and reflection
Lab safety information for student and teacher
Red Fluorescent Protein
A.
B.
C.
D.
Restriction (cut the rfp gene out)
Ligation (put the rfp gene into a plasmid)
Confirmation
Transformation (put the plasmid with rfp into a
bacteria)
E. Culture the bacteria, isolate and purify the protein
Procedures Involved
Complete
GE
Abridged
GE
Focus on
Bacteria
1. Practice Pipetting
X
X
2. Restriction
Analysis
X
X
3. Ligation
X
4. Confirmation
X
X
5. Transformation
X
X
X
6. Culture of E.
coli/Purification
X = included; * = optional
X
*
*
X
Intro to
Biotech
X
Lab Sequences
Saturday
◦ Introduction
◦ What are the ABE labs?
◦ Lab 1.1 & 1.2: How To Use a Micropipette and Gel
Electrophoresis
◦ Lunch
◦ Lab 5A: Transforming Bacteria with the pARA-R Plasmid
October 12, 2013
Sunday
◦ Lab 2a: Preparing to Verify the RFP Gene: Digesting the
pARA-R Plasmid
◦ Lab 4a: Verification of the Recombinant Plasmid Using Gel
Electrophoresis
◦ Lunch
◦ Lab 4a: Verification of the Recombinant Plasmid Using Gel
Electrophoresis (cont.)
◦ Lab 5A: Transforming Bacteria with the pARA-R Plasmid
(cont.)
October 13, 2013
Red Fluorescent Protein
A.
B.
C.
D.
Restriction (cut the rfp gene out)
Ligation (put the rfp gene into a plasmid)
Confirmation
Transformation (put the plasmid with rfp into a
bacteria)
E. Culture the bacteria, isolate and purify the protein
Procedures Involved
Complete
GE
Abridged
GE
Focus on
Bacteria
1. Practice Pipetting
X
X
2. Restriction
Analysis
X
X
3. Ligation
X
4. Confirmation
X
X
5. Transformation
X
X
X
6. Culture of E.
coli/Purification
X = included; * = optional
X
*
*
X
Intro to
Biotech
X
Lab Sequences