Transcript IGEM-_Chemotaxis_July_21

IGEM- Chemotaxis
July 15-21
Dose Dependant Swim Assay
• Goal:
– To measure the chemotactic response of bacteria
to differing concentrations of toluene.
• Procedure:
– Plate a small number of bacteria onto center of a
swim plates containing differing concentrations of
toluene.
– Measure the distance that the bacteria
swim/grow in a 14 hour incubation period.
Problems
• Agar at the .15% concentration is EXTREMELY
fluid. We could not invert plates for incubation
or measurement/photgraphing.
• Toluene is flammable and volatile.
• Plating a consistent number of bacteria onto
the swim plate is difficult with an inoculating
loop
Results
Average Area (mm)
pWW0
10mM
0.335
5mM
0.550
0mM
0.168
KT2440
10mM
5mM
0mM
0.507
0.528
0.104
Further Research
• Use .3% agar (I calculated incorrectly)
• Grow the colonies out in media, take an
absorbance reading, and plate a small amount
of a dilution to achieve a similar initial cell
count among plates.
• Larger # in order to verify results which do not
agree with paper we used as guide.
• Higher/Lower Concentrations
• Gradiants