Transcript 슬라이드 1 - G

Is your drinking water safe?
Please check with “Drinking Water Hometest Series”
Drinking Water Hometest suggest simple
and easy drinking water safety monitoring by
self-conducting bacterial test in home. The
protocol is very simple and the principle,
color change of water sample induced by
bacteria’s specific enzyme reaction, is
recommended as official test method for
water safety test in many countries.
▶ Specific enzyme reaction coupled with color change!
(for Coliforms & E. coli)
▶ Easy protocol and convenient testing!
Simple
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Rapid
detection
Easy
reading
High
reliability
Safe
wasting
Even if you are not a scientist, you can test bacterial safety of water sample by yourself
with our “Drinking Water Hometest” because the protocol is very simple and everything’s
prepared in kits.
You can read the result within 18 hrs to 24 hrs.
You can achieve reliable test result with our principle, so called “color change of water
sample induced by bacteria-specific enzyme reaction” is recommended as official test
method for water safety test in many countries.
It is so sensitive and reliable as even one bacterium is detected.
We provide safe wasting of the test materials with Bacterzero, natural-origin bacteria
sterilization reagent. After testing add Bacterzero into your water sample and prohibit
secondary contamination.
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Detection of total aerobes
Detection of coliforms
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Detection of coliforms and
E. coli simultaneously
Drinking Water Hometest
BacterView for total aerobes
BacterView is a kit detecting total aerobic bacteria in your drinking water, which change the
color to red. Total aerobic bacteria indicate contamination or stability of water and exist widely in
river, ground water, soil, foods, air and so on. They grow in the presence of oxygen and too many
numbers can cause stomach disorder or diarrhea.
Specification
Cat. No.
Product
Description
Q/T
2 tubes
BcaterView Medium (4 mL)
HW001
Packing
BacterView BacterView Substrate (1 mL)
2 tests/box
2 vials
1 ea
BacterZero (4 mL)
Principle
Redox dye (2,3,5-triphenyl tetrazolium chloride, TTC)
is converted to formazan because of reduction by
dehydrogenase of bacteria, which indicates red color.
Triphenyltetrazolium
chloride
Reduction by
Dehydrogenase
Test Procedure
1
Add 20 mL sample
to a medium tube
with a filled volume
of 4 mL.
2
Add 1 mL substrate
to the medium tube
filled with sample
plus medium.
3
Incubate the sample
with medium at
35~37℃ for 16~24
hrs. (at ambient
temp. for 36 hrs.)
for
total
aerobes
Positive
for
total
aerobes
If aerobic bacteria exist in
your drinking water, the
color of the medium will be
changed to red slowly. The
color concentration depends
on the number of aerobic
bacteria.
5
4
Read the
result
Interpretation of Result
Negative
Formazan
Add 2 mL of BacterZero
to the test-finished tube
and mix. Leave for 30
min. and discard it inside
into a toilet.
Storage condition and
Shelf life
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Dark place, 4~25℃
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6 months after manufacture
Drinking Water Hometest
ColiView for coliforms
Coliview is a kit testing presence and absence of coliforms in your drinking water allows specific
enzyme-chromogenic substrate reaction. Coliforms are important indicators of fecal contamination.
If coliform bacteria exist (blue color), you should enhance hygiene practice because the result
provide a possibility of other food poisoning pathogens.
Specification
Cat. No.
HW002
Product
ColiView
Description
Q/T
ColiView Medium (4 mL)
2 tubes
ColiView Substrate (1 mL)
2 vials
Packing
2 tests/box
1 ea
BacterZero (4 mL)
Principle
If coliforms are present, they use the enzyme βgalactosidase (produced by all coliforms, including
E.coli) to metabolize the specific color-producing
substrate, X-gal and the medium changes color from
near colorless to blue.
X-gal
β-galactosidase
Test Procedure
1
Add 20 mL sample
to a medium tube
with a filled volume
of 4 mL.
2
Add 1 mL substrate
to the medium tube
filled with sample
plus medium.
3
Incubate the sample
with medium at
35~37℃ for 16~24
hrs. (at ambient
temp. for 36 hrs.)
Read the
result
Interpretation of Result
Negative
for
coliforms
Positive
for
coliforms
After incubation, the color
of medium in reaction
bottle would be changed to
blue under presence of total
coliforms in sample water.
On the other hand, color of
medium would not be
changed under absence of
total coliforms in sample.
5
4
Add 2 mL of BacterZero
to the test-finished tube
and mix. Leave for 30
min. and discard it inside
into a toilet.
Storage condition and
Shelf life
●
Dark place, 4~25℃
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6 months after manufacture
Drinking Water Hometest
E.ColiView for coliforms & E. coli
E.Coliview use specific enzyme-chromogenic substrate reaction to simultaneously detect total
coliforms and E.coli from home and environmental water samples. Coliforms including E. coli are
important indicators of fecal contamination. If they exist (blue color) in your drinking water, you
should enhance hygiene practice of it because the result provide a possibility of other food
poisoning pathogens.
Specification
Cat. No.
HW003
Product
Description
E.ColiView
Q/T
Packing
E.ColiView Medium (4 mL)
2 tubes
E.ColiView Substrate (1 mL)
2 vials
2 tests/box
1 ea
BacterZero (4 mL)
Principle
If coliforms are present, they use the enzyme βgalactosidase to metabolize X-gal and the medium
changes color from near colorless to blue. If E.coli are
present, they use β-glucuronidase (produced by E.coli
strains but not by general coliforms) to metabolize
MUG and create fluorescence.
X-gal
MUG
β-galactosidase
β-glucuronidase
Test Procedure
1
Add 20 mL sample
to a medium tube
with a filled volume
of 4 mL.
2
Add 1 mL substrate
to the medium tube
filled with sample
plus medium.
3
Incubate the sample
with medium at
35~37℃ for 16~24
hrs. (at ambient
temp. for 36 hrs.)
5
4
Read the result
Add 2 mL of BacterZero
to the test-finished tube
and mix. Leave for 30
min. and discard it inside
into a toilet.
Interpretation of Result
Negative
Positive
for
for
coliforms coliforms
Positive
for
E. coli
Coliform-positive reaction turns the
medium blue. The blue color means
that total coliforms are present in
the sample. If no color change
means that there are no presence
of total coliforms in the test sample.
E. coli-positive reaction caused the
medium to fluorescence under a
long-wavelength UV lamp (365nm).
If a bright blue fluorescence is
observed, this indicates that MUGpositive E. coli bacteria are present.
Storage condition and
Shelf life
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Dark place, 4~25℃
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6 months after manufacture
Product Description
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In Vitro Diagnostic Kit, 2 Tests.
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Keep out of reach of children so that they might not eat.
Keep storage conditions and use the product within the expiry date.
We cannot guarantee the performance if you do not follow the instructions.
(Date of manufacture is indicated on a vacuum packed plastic bag)
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Be sure to dispose using BacterZero after testing.
※ For your reference!
Excellent bactericidal Effect of BacterZero
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Escherichia coli
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Control
Control
(No BacterZero)
Dilution Rate
(No BacterZero)
10-3
10-4
10-5
Test
10-4
10-5
5 min.
20 min.
60 min.
After
5 min.
After
20 min.
After
60 min.
No BacterZero
2.89×108
2.89×108
2.89×108
0
Addition of
BacterZero
0
0
0
99.999%
Sterilization (%)
99.999%
99.999%
99.999%
20 min.
60 min.
After
5 min.
After
20 min.
After
60 min.
No BacterZero
3.45×108
3.45×108
3.45×108
Addition of
BacterZero
0
0
Sterilization (%)
99.999%
99.999%
Time
10-3
(Addition of
BacterZero)
5 min.
Description
Dilution Rate
Test
(Addition of
BacterZero)
Time
Bacillus cereus (Spore-forming bacterium)
Time
Time
Description