Transcript staph

- GRAM +VE BACTERIA:1- Staphylococcus aureus
2- Streptococcus agalactiae
3- Streptococcus dysgalactiae
BACTERIAL CAUSE
*GRAM +VE BACTERIA
1-Staphylococcus aureus
Taxonomic lineage
Kingdom: Eubacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Staphylococcaceae
Genus: Staphylococcus
Species : S. aureus
MORPHOLOGY
Gram-positive coccus
grape-like clusters
golden-yellow colonies
PATHOGENICITY
1-HAEMATOGENIC PATHWAY
2-LOCAL
Lab. Diagnosis
SAMPLING MILK SAMPLE
A-Media
BROTH MEDIA:- produce turbidity
NUTRIANT AGAR :- produce smooth rounded golden yellow colonies
BLOOD AGAR :- produce clear zones of b haemolysis
MANNITOL SALT AGAR
POLYMYXIN AGAR
VOGOL JOHNSON MEDIA
B-Biochemical identification
catalase :-positive
coagulase test :-positive
lipase :-a yellow color and rancid odor smell
DNAse :-zone of clearance on nutrient agar
phosphatase :-a pink color
C-Virulence factors:Toxins:Pyrogenic toxin superantigens
Exfoliative toxins
Staphylococccal toxins that act on cell membranes include alphatoxin, beta-toxin, delta-toxin
Treatment
penicillinase IS PRODUCED SO PENICLLIN NOT USED
methacillin
2- Streptococcus agalactiae
Taxonomic lineage:
Kingdom: Bacteria.
Phylum: Firmicutes.
Class: Bacilli.
Order: Lactobacillales.
Family: Streptococcaceae.
Genus: Streptococcus.
Species: Streptococcus agalactiae group
Groups: A,C &G
Morphology:
Gram-positive cocci
Non motile
Non spore forming
Aanaerobic & facultative anaerobic
Pathogenisis:
Streptococcus agalactiae breaks the natural barriers of
the udder, enters the teat canal, and ascends in the milk
through the quarter
Lab. Diagnosis:
1-Sampling: milk sample
MILK SAMPLE
2-Direct Electron Microscope
3-Isolation&Identification:
A-Media:
1- Liquid media
2- blood agar media
3- MacConkey agar:
4- Biochemical Identification:
1-Catalase: -ve.
2-Coagulase: -ve
3- Esculin reaction: -ve.
4- Hippurate hydrolysis test:+ve
5- CAMP Test
Treatment
Penicillin
Streptococcus dysgalactiae
Kingdom: Bacteria.
Phylum: Firmicutes.
Class: Bacilli.
Order: Lactobacillales.
Family: Streptococcaceae.
Genus: Streptococcus.
Species: Streptococcus dysgalactiae
Morphology
Gram-positive cocci
long chains in growth medium
non motile
non spore forming
anaerobic & facultative anaerobic
Pathogenisis
Streptococcus dysgalactiae breaks the natural barriers
of the udder, enters the teat canal, and ascends in the
milk through the quarter
Lab. diagnosis:
A-Media:
1-Liquid media
2-Solid media
3-Blood agar:
4-Selective media
5- Edward’s media
6- bile esculin agar
7-MacConkey’s agar
8- Growth media containing 6% NaCl.
B- Biochemical identification
1-Catalase: -ve.
2-Coagulae: -ve.
3-Peroxidase: -ve.
4-Nitrate reductase: -ve
5-Camp test: -ve
6-Vogus Proskauer: -ve
7-Arginine reaction; +ve.
C- Antigenic structure
Bacterial superantigens are protein toxins with an ability to cause serious
diseases in animals by activating a large number of T cells. Crystal structure of S.
dysgalactiae-derived mitogen (SDM) showed that it is a novel superantigen that is
distinct from other known superantigens based on phylogenetic analysis.
Treatment
Penicillin
GRAM-VE BACTERIA:1- Escherichia coli
Phylum: Proteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
Genus : Escherichia
Species: E. coli
MORPHOLOGY
Gram-negative
facultative anaerobic
non-sporulating.
pathogenicity
Escherichia coli causes inflammation of the mammary gland
in dairy cows around parturition and during early lactation with
striking local and sometimes severe
Lab. Diagnosis
A-Media
E. coli OD Media
•Rich growth media
•Easy to handle
•High isotopic purity
•Require minimal optimization adjustments
•Ensure high expression yields
B-Lab diagnosis
1)cultivation on
Blood agar: hemolysis or non hemolysis.
Mac Conkey agar : bright pink colonies
Eosin methylene blue agar
2)microscopical examination test
Gram (-ve) _:medium in size_:singly arranged_:Non sporulated
c- Biochemical test
a)oxidase : (-ve).
b)Urease activity : (-ve).
c)Catalase :(+ve).
d)Nitrate reduction :(+ve).
(IMVC test)
a)indole production (+ve).
b)methyle red test : (+ve).
c) Vogus Proskauer: (-ve).
d)citrate utilization test :(-ve).
TREATMENT
amoxicillin
B.FUNGAL CAUSE:1-Aspergillus
Kingdom:Fungi
Phylum:Ascomycota
Class:Eurotiomycetes
Order:Eurotiales
Family:Trichocomaceae
Genus:Aspergillus
aerobic : fungi grow on carbon-rich substrates
LABORATORY DIAGNOSIS
SAMPLING:- MILK SAMPLE
a)Direct microscope examination
b) isolation and identification
Treatment
Removal of blockages in reaction and improvement of cell respiration
Modulation of the immune system
Cleansing of the bacteriological soil
Cryptococcus neoformans
Morphology
-
C .neoformans grows well at 37˚C
sabouraud dextrose agar
appears as soft , creamy , opaque colony in 3-5 days
becomes mucoid and creamy to tan
Pathogenesis
Mastitis is the most comman infecation in catall ,which is characterized by hardness and
swelling of theaffected quarters and grayish white secreations.
Laboratory diagnosis
A-Sampling : milk sample
B-Direct microscopic examination
+ve samples stained with India ink
C-Culture characters
- Culture onto sabouraud dexterose agar without cycloheximide, incubated 37ºC
- Show creamy moist colonies.Bown coloured colonies are clear on Niger_seed
medium.
- fermentation :none
D-Biochemical reacations
- nitrogen assimilation : nitrate:-ve
tryptone:+ve
- carbon assimilation : Glucose : +ve
Galacatose : +ve
Maltose : +ve
Lactose :-ve
Sucrose : +ve
Cellobiose : +/w
Raffinose : +/w
Inuline :- ve
D-xylose : +ve
TREATMENT
IMIDAZOLS
Candida albicans
Morphology
- sabouraud dextrose agar
- C. albicans developes within 24 to 48 h
- raised creamy ,opaque colonies of 1-2 mm in diameter
Pathogenesis
C. albicans is normally present in small numbers in female genital tract . The
colonization - is aided by the ability of C. albicans to adhere to mucosal cells .
Laboratory diagnosis
A- sampling : milk sample.
B- Direct microscopic examination :
of 10% KOH preparation or stained films by Gram,PAS.etc.
C-Culture characters :
-Culture onto sabouraud dexterose agar
D-Biochemical reacations:
fermentation : Glucose : +ve
Galacatose : + ve
Maltose : +ve
Lactose :- ve
Sucrose: +/Cellobiose : -ve
Raffinose: - ve
Inuline :- ve
Assimilation : Glucose : +ve
Galacatose : +ve
Maltose : +ve
Lactose :-ve
Sucrose : +ve
Cellobiose : -ve
Raffinose : - ve Inuline :- /+
Starch : +ve
D-xylose : +ve
TREATMENT
IMIDAZOLS