Transcript Cell Structure & Function Inquiry Lab

Cell Structure & Function Inquiry
Lab
Lab Packet
1. Ocular,
Eyepiece
2. Body tube
3. Arm
4. Revolving nosepiece
5. Low-power objective
6. High-power objective
7. Objective lenses
8. Stage clip
12. Coarse adjustment knob
13. Fine adjustment knob
14. Base
9. Stage
10. Diaphragm
11.
Light source
• Prepared Slides: slides that
are purchased from a
company and are labeled,
stained, and ready for viewing.
They DON’T require a cover
slip. Used for viewing dead
cells and organisms.
• Blank Slides: slides that you
must prepare in order to view
live specimens. Usually
requires you to make a wet
mount. Also REQUIRES a cover
slip
Blank Slide
Cover Slip
Wet Mounts
2
Making a Wet Mount
• Obtain a blank slide
• Use forceps or a pipette to
place your specimen in the
middle of the slide
• Use pipette to add 1 drop of
water (if specimen isn’t
already contained in a liquid
such as pond water)
• Cover with a cover slip
1
3
4
How to Focus a Microscope
1. Always start with Low Power
•
•
•
Place the slide on the stage and fasten with
stage clips
Rotate objective lens to low power (smallest)
Use course focus knob to bring image into focus
2. Medium Power
•
•
Rotate objective lens to medium power (2nd smallest
objective)
Use course focus knob to bring image into focus
3. High Power
•
•
Rotate objective lens to high power (largest objective)
Use FINE FOCUS knob to bring image into focus
NEVER USE COURSE FOCUS KNOB WHEN IN HIGH
POWER BECAUSE IT CAN DAMAGE THE
MICROSOCOPE!!!!!
Cheek Cells
Yogurt Cells
Yogurt
Bacteria
Bacterial Highly Magnified
They come in different
shapes (Rod, Coccus) and
may travel alone or in
groups
Eukaryotic & Prokaryotic Cells
Cell
Membrane
Animal Cell
Plant Cell
Parts 2 & 3
• In this section you will be using your
smartphone and/or the class computers to
look up information.
• Be sure to take notes on the internet sources
you use because you will be REQUIRED TO
CITE THEM in MLA format in Part 3
• Directions for MLA citations are on the next
page.
MLA Format – In-text Citations
•
WEB SITES: There are times when some elements of information about a source
will be missing. For Web and periodical articles, an author’s name is sometimes
omitted because the authorship is considered to be the organization hosting or
publishing the article.
– For corporate authorship, include the name of the organization.
• Example 1: When the polishing error was discovered, the Hubble mirror was described as
“needing glasses” (NASA 234).
– For Web sites that lack page numbers, do not make them up or use the page numbers
on a printout. List only the author’s last name.
• Example 2: The award is based on the results of Web-based surveys, limiting the survey sample
to those who have Internet access (Doe).
– No human or corporate author for a Web article, use an abbreviated title as the
reference. Use the first part of the Web article title, so that it refers clearly to the title in
the Works Cited. For an article titled, “New Research Shows Americans Getting Too Little
Vitamin D,” the in-text citation might look like this:
• Example 3: New studies have concluded that Americans are getting an insufficient amount of
Vitamin D, in part because of too little time spent outdoors (“New Research”).
– If you must list the website name in the signal phrase that appears before a quote in
your text, - do not include URL addresses in-text. Provide partial URL’s, like MSNBC.com
not http://www.msnbc.com.
Sperm Cell
Neuron
Root Cell
Parenchyma Cell
Bacillus Bacteria
Spirochete Bacteria
Euglena
Dinoflagellate