Transcript Parts Registry Scavenger Hunt

Max Showalter
7. June 2012
Purdue University
Let’s begin at the parts registry homepage
When you get to this webpage, you will see several
different icons you can click on.
Click On
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The first thing you’ll notice on this page is a
long list of Promoters, Ribosome Binding
Sites, etc…
Further down, you will see that you can
browse parts by part, type, function, or
chassis
From this page and the search bar in
the top right your job will be to find
the pieces for a functional device.

If I’m going to request a part for my iGEM
Team…
 What email address do I send my request to?
 What do I include?

If I make a BioBrick part:
 What will the suffix be?
 What will the suffix be if I use Spe1 to cut my
plasmid?
 What temperature should I do my digestion if I am
using Spe1?

Find the part(s) that stopped transcription in
the banana smell generating device (You will
have to find the banana smell generating
device first!)
 What are these parts called?
 What are their part registry numbers?
 How many base pairs does each have?

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Find a constitutive eukaryote promoter that
works and has a registry star
Find a Kozak sequence for yeast that works
and has a registry star
A protein that will make your yeast fluoresce
cyan – [Cyan Fluorescent Protein]
A terminator that works in yeast
(What is the name of the Chassis for these
Now It’s Time to Assemble a
Part
All Biological Circuits must have:
1. Promoter
2. Ribosome Binding Site (RBS)
3. Coding Region
4. Terminator
Use Only:
1. Parts that work
2. Parts that are available
Specify a chassis and ensure your parts
match this chassis
Rules to the
Game
Let’s Make a Metal Sensor..
Fe
Metal Ions
Fe
Fe
Device


Assemble a list of the parts required for the
device
Follow all the rules given
Team A
Team B
Develop two systems
for E. coli that will:
1. Make the
bacteria fluoresce
cyan
2. Make the
bacteria smell
like winter mint
in the presence of
salicylic acid
Develop two different systems for
S. cerevisiae that will
1. Make the yeast fluoresce
Yellow
2. Make the yeast fluoresce
orange
As a bonus: yeast (a eukaryote)
doesn’t use a ribosome
binding site in the same sense
as prokaryotes. What is the
analogous part in yeast?
Describe how it is like an RBS.

Make a double tiered system that will turn off
color when banana smell has been turned on