Transcript Triclosan Lab Protocol 13-14 Hon Bio

Natural Antimicrobials
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Cinnamon
Cumin
Green Tea
Oleupurin (olive
extract)
• Turmeric
• Coleus Oil
Triclosan
• Developed in 1972
• Kills bacteria by
inhibiting synthesis of
fatty acids for cell
walls
Increased Use of Triclosan
• Found in 36 products in
1975
• Present in 700+ products
in 2010
• 1 billion/yr industry
• Soaps, toothpaste,
lockers, razors, lip balm,
sandals, deodorant,
children’s toys
Overuse?
• Can hasten the
development of drugresistant strains of bacteria
• Shown to cause abnormal
weight loss, brain tumors
and irregular limb growth
in amphibians
• One of most common
contaminants in river
water
• Stored in mammalian fat
Assessing the Relative Efficacy of
Triclosan-based Antimicrobials
• Purpose: To determine the relative efficacy
of Triclosan v. Non-Triclosan-based
antimicrobials in inhibiting the growth of
bacterial present on human skin
• Method: Your task is to design a valid
protocol for the assessment of this question
in a controlled fashion with minimal
confounding variables
Available Materials
• Antimicrobials
• Nutrient agar culturing
dishes (2 per group)
– Triclosan Handsoap
– Non-triclosan
• Sterile cotton swabs
handsoap
• Forceps
– Alcohol-based sanitizer
• Probes
– Clorox sanitary wipes
• Hole Punches
(ammonium chloride
based)
• Paper Towels
– Hydrogen Peroxide
• Goggles
– Concentrated salt water
• Incubators
– Pure Water
– 25 C
– 37 C
Safety Precautions
• All members must wear goggles while at
lab table
• Do not culture or use antimicrobials on any
mucosal membranes, ear or eye tissues
• Dispose of all used cotton swabs in a lined
garbage can