Transcript Folie 1 - Prontosan&#174

What is a biofilm?
A biofilm (literally = living thin layer) consists of a community of micro-organisms of various species, which
are bound in a three-dimensional matrix of Extracellular Polymeric Substances (EPS). The EPS consist
largely of sugar compounds.
Biofilms form on a wide variety of surfaces and materials, e.g. on living tissue such as wounds, invasive
indwelling instruments and in artificial or natural aquatic systems.
As biofilms are bound firmly to these surfaces, they cannot be removed by simple rinsing.
Characteristics features of biofilm are their increased resistance to many antimicrobials substances, to drying
out and to the defense mechanism of the host organism.
Acute or chronic wounds are susceptible to infection because micro-organisms in the form of a biofim can
invade the wound milieu. As soon as a wound is contaminated with micro-organisms, and especially if these
have formed a biofilm, wound healing is impaired..
In a recent study by James et al (2007) it found that in 60% of chronic wounds a biofilm is present.
Once a biofilm is disturbed & removed a wound can heal quicker
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The formation of a biofilm takes place in several phases
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(I) Biofilm building - bacteria adhere and replicate to a surface
flow direction
Surface
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(II) Building up a biofilm
flow direction
Surface
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(III) Bacterial Growth inside the biofilm
flow direction
Surface
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(IV) Bacteria build up a biofilm to protect themselves from enemies
flow direction
Surface
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(V) Release of bacteria, leading to renewed colonisation elsewhere
and again bacteria replication
flow direction
Surface
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The problems a biofilm creates
Once a biofilm is disturbed & removed a wound can heal
quicker
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Prontosan – the answer to biofilm removal
The usually applied solutions for the irrigation of
wounds (saline, water etc)
glide over the biofilm without removing it
PRONTOSAN is able to remove the biofilm by
destroying it’s structure
Prontosan contains betaine which is a
surfactant and this physically removes
biofilm this making wound healing possible.
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Clinical Evidence
Study
Summary
Seipp H M, Hofmann A,
Hack A, Skowronsky A,
Hauri A, (2005 – 2007)
Efficacy of various
wound irrigants against
biofilm
Kaehn K,Eberlein T,
(2009) In-vitro test for
comparing the efficacy
of wound rinsing
solutions
James A, (2007)
Biofilms in chronic
wounds
This study tested Prontosan, Saline and Ringers in there efficacy against biofilm of
Pseudomonas Aeruginosa. Baseline biofilms were unaffected by Ringers and Saline
but Prontosan reduces biofilm with no adverse affects enabling healing to take place.
The aim of this study was to test the efficacy of Saline, Ringers, Prontosan and
Ocenisept to solubilize and remove wound coatings using a wound coating model.
Prontosan was the only solution were the test coatings disintegrated and the denatured
proteins solubilized. The test coatings became fixed and insoluble when immersed in
Octenisept.
Light and scanning electron microscopy techniques were used to analyze 50 chronic
wounds and 30 were characterized as containing biofilms (60%). Molecular analysis
was performed on the remaining 27 chronic wounds, this revealed diverse
polymicrobial communities and the presence of bacteria.
Phillips PL, Wolcott RD, This article describes what biofilms are and the important roles they play in disrupting
Fletcher J, Schultz GS, wound healing. In addition, it discusses potential interventions (Prontosan) aimed at
(2010)
removing / reducing biofilms and preventing their reformation in wounds.
Biofilms made easy
TO READ FULL STUDIES – PLEASE GO TO THE CLINICAL EVIDENCE SECTION
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