Transcript Baikal’s pelagic environment

Baikal’s pelagic
environment
E. Silov
M. Bölter
D. Fleischer
M. Givetiev
X. Glushenok
A. Mokryy
Y. Schweikhard
First day 24th August
• Discussion of possible
sampling and analysis
methods
• Sampling preparations
like sampling gear
• Microbiological sampling
of the surface waters in
the harbour of Bol’shie
Koty
Microbiological lecture
• Introduction of the students to different procedures
and methods of aquatic microbiological science
• Background information
– Advantages and disadvantages of stain methods with
special dyes (DAPI and Acredinorange)
– Sampling processing
Introduction to phytoplankton
laboratory
• Introduction to the processing of Phytoplankton
samples
• Explanation of Chlorophyll a extractions from
samples from Point#1
Microbiological analysis
• Preparation of Agar for
growth experiments and
colony forming bacteria
• Epifluorescence
microscopy
– Staining samples with
Acredineorange and
DAPI
– Practicing counts of
bacteria individuals by
epifluorescence
microscopy
– Getting calculations done
Microbiological survey
Bacterial counts from Bol'shie Koty
N u m b e r o f o rg a n ism s
1,E+06
1,E+06
8,E+05
6,E+05
4,E+05
2,E+05
0,E+00
Surface Point#1
250m Point#1
Sample
Pipe
Microbial growth experiment
Number of organisms
Growth of Point#1 250m bacteria
population
5,0E+05
4,5E+05
4,0E+05
3,5E+05
3,0E+05
2,5E+05
2,0E+05
1,5E+05
1,0E+05
5,0E+04
0,0E+00
26,840
25.08.
26.08.
Day
Zooplankton community of
Lake Baikal
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Epischura baicalensis
Notholca intermedia
Keratella cochlearis
Keratella quadrata
Kellicottia longispina
Filinia terminalis
Synchaeta sp.
Daphnia longispina
Cyclops kolensis
Cruise to Maloe More
Maloe More Samples
N u m b e r o f o rg a n is m s
2,0E+06
1,8E+06
1,6E+06
1,4E+06
1,2E+06
1,0E+06
8,0E+05
6,0E+05
4,0E+05
2,0E+05
0,0E+00
Benthic Sediments
Surface (Acridine orange)
Samples
Surface (DAPI)
Thank you
for your
attention!