Transcript Alta, Norway, Sept, 6th - 8th, 2010: The 7th Circumpolar

Presentation of ALGEBAKT project.
Antibacterial effect of arctic macroalgae species
DMV, PhD, Margarita Novoa-Garrido
Alta, Norway, Sept, 6th - 8th, 2010: The 7th Circumpolar Agricultural Conference
"Circumpolar Agricultural and Land Use Resources - Prospects and Perspectives for Circumpolar
Productions and Industries"
The MABIT-programme is an industrial
R&D program for Northern Norway.
Bioforsk Nord Bodø.
Bioproduction and ecosystems along the coast
Coastal ecology - possibilities for
commercial exploitation of coastal and
marine resources without damaging
ecosystems.
Our main focus is the algae.
Background for the project
• Antibiotics: therapy and growth
promotion
• Ab-resistance
• Antibiotics banned in Europe as
antibiotic growth promoters since
2006
It was on a short-cut through the hospital kitchens that Albert was first
approched by a member of the Antibiotic Resistance.
Antibiotic resistance test of
P. aeruginosa strain
Foto: Unni Lorentsen
A. nodosum
Pelvetia canaliculata
Palmaria palmata
Alaria esculenta
ALGINATE
Vegetables in seaweed project in
Bioforsk Nord.
Foto: I. Øvsthus
A. nodosum
L. hyperborea
SEAWEED
MEAL
Sheep in seaweed meal project in
Bioforsk Nord.
Foto: H.J. Skulstad
Algae composition
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Dietary fibre (<8% DM)
Protein (5-10 % DM)
Fat (2-4 % DM)
Carbohydrates (45-60 % DM)
Vitamines: A, C, E, B1, B2, B3, B8, B9
Minerals
Secundary bioactive metabolites
Macroalgae biologiske effekter
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Antibakterial
Antivirus
Antitumor
Antioxidant
Antiinflammatory
Immune stimulans
Growth stimulant
Stomach up to 102
Lactobacilli,
streptococci, yeast
Animal health
Jejunum and ileum
104-106 CFU/ml
Lactobacilli, coliforms,
Streptococci, fusobacteria,
clostridia, bacteriodes,
bifido bacteria
Environment
Immune
system
Bacterial
population
Diet
Colon 1010-1012 CFU/ml
Lactobacilli, bifido
bacteria, eubacteria,
fusobacteria, coliforms,
clostridia, veillonella,
yeasts
Photo: Birger Volden
Some research results
• Animal husbandry: nr. of E. coli intestine,
shedding of E. coli O157 and Salmonella, nr.
adherent Lactobacillus sp. Innate immunity and B
lymhocyte activity.
• Aquaculture: Antibacterial effect on fish pathogens
such as Vibrio sp. and Aeromonas sp.
• Furanones, organohalogens, floratannins, fucoidans,
haloperoxidases, alginate
Aims of the project
• Generate knowledge about bioactive components
with antibacterial effect in macroalgae species
adapted to the northern Norwegian climate to get a
better overview of the potential for developing new
products based on their antibacterial property.
Material and methods
• Bacteria sheep: Lactobacillus sp., Enterococcus
faecium, Enterococcus faecalis, different E. coli strains
• Bacteria fish:Vibrio sp., Pseudoalteromonas sp.,
Aeromonas sp.
• Macroalgae: Four red species and two brown species
• Minimum inhibitory concentration (MIN)
• Liquid chromatography-mass spectrometry
Synergy with other ongoing prosjects
• «Natural sources of antioxidants – a necessity for
animal health and welfare and product quality in
organic livestock production.»
• The research Council of Norway.
• Effekt of feeding macroalgae on the milk quality of
dairy cattle.
• Effekt of feeding macroalgae on intestinal bacterial
flora and immune system of sheep.
Future visions
• Screening
• Identification of biologically activ components
• Animal experiments
THANK YOU FOR YOUR ATTENTION!