Transcript The Effects of Fruit Juices and Ampicillin on Escherichia coli

THE EFFECTS OF FRUIT JUICES
AND AMPICILLIN ON
ESCHERICHIA COLI
Kelly Mannion~ Academy of Notre Dame
Question
If taking water with ampicillin is
replaced by fruit juice will it interfere
with the medicine’s effectiveness
against E. coli?
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Background and Rational
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Juices are rich in antioxidants
Escherichia coli (E. coli) - gram-negative bacteria
that lives in people’s intestines that can cause
infections
Ampicillin- a form of penicillin that fights or
prevents the growth of the bacteria in a person’s
body
Acidic fruit juices can harm antibiotics
Rationale
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This investigation was designed to test
and find out the effects of ampicillin
on E. coli when combined with fruit
juice as compared to water.
Hypothesis
If juice is added to ampicillin, then
the citric juices will hinder the
effectiveness of the ampicillin against
E. coli
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Procedure
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Dip 3 sterile disks in orange juice, 3 sterile disks in pomegranate
juice, 3 sterile disks in cranberry juice, 3 sterile disks in grapefruit
juice
Dip 3 sterile disks in sterile, distilled water and 3 in ampicillin and
allow all to dry.
Mix 5 solutions consisting of 1mL of ampicillin and 5mL of each
juice and sterile water.
Allow 3 sterile disks to absorb in each solution and dry in
separate petri dishes.
Pipette 1ml of E. coli on a petri dish with a nutrient agar and
place a sterile disk for each juice on it.
Do the same for another petri dish and place 4 sterile disks on it
(ampicillin, ampicillin and orange juice, ampicillin and cranberry
juice, ampicillin and grapefruit juice).
Procedure (continued)
 Pipette
1ml of E. coli on another petri dish and place 3
sterile disks on it (water, ampicillin and pomegranate
juice, ampicillin and sterile distilled water).
 Seal and label the petri dishes accordingly and place
in an incubator.
 Repeat steps 6-7 for 2 additional trials.
 Allow the petri dishes to stay in the incubator for 48
hours then remove and observe.
 Record the zone of inhibitions from one side to the other
for each sterile disk (mm) and make observations.
Variables
Independent: Fruit juice used (Cranberry,
Pomegranate, Grapefruit, Orange)
 Dependent: Zone of inhibition
 Control: Sterile water, ampicillin
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Data
The Average Effects of Fruit Juices+Ampicllin on E. coli
30
Zone of Inhibition (mm)
25
20
15
10
5
0
AMPICILLIN
-5
ORANGE +
AMPICILLIN
CRANBERRY +
AMPICILLIN
POMEGRANATE +
AMPICILLIN
GRAPEFRUIT +
AMPICILLIN
STERILE WATER +
AMPICILLIN
Data
The Average Effects of Ampicillin, Orange Juice, and Ampicillin +
Orange Juice on E. coli
30
Zone of Inhibition (mm)
25
20
15
10
5
0
Ampicillin
Orange Juice
Orange Juice + Ampicillin
Data
The Average Effects of Ampicillin, Cranberry Juice, and Ampicillin +
Cranberry Juice on E. coli
30
Zone of Inhibition (mm)
25
20
15
10
5
0
Ampicillin
Cranberry Juice
Cranberry Juice + Ampicillin
Data
The Average Effects Of Fruit Juice, Water, and Ampicillin on E. coli
30
Zone of Inhibition (mm)
25
20
15
10
5
0
ORANGE
CRANBERRY POMEGRANATE GRAPEFRUIT STERILE WATER
AMPICILLIN
Conclusion
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Fruit juices and sterile water do affect both the
growth of E. coli and the ampicillin
No statistical difference between the groups of
data collected
No fruit juice can be determined as the most
effective or harmful in aiding the ampicillin
Limitations
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One petri dish had to be discounted-inconsistency in
the data for each of the pomegranate, grapefruit,
and sterile water mixtures with ampicillin
Possibility that the petri dishes were not coated
evenly
Various incubation times
References
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Cape Cod Cranberry Growers’ Association. (2011). History of Cranberries [The history of cranberry juice]. Retrieved October 18, 2011, from Cape Cod Cranberry Growers’ Association website:
http://www.cranberries.org/cranberries/history.html
Centers for Disease Control and Prevention. (2011, July 8). Escherichia coli O157:H7 and other Shiga toxin-producing Escherichia coli (STEC) [Frequently asked questions on E. coli]. Retrieved October 18, 2011, from Centers for Disease
Control and Prevention website: http://www.cdc.gov/nczved/divisions/dfbmd/diseases/ecoli_o157h7/
Cerner Multum, Inc. (2010, December 15). Ampicillin [Ampicillin medical facts]. Retrieved October 18, 2011, from Drugs.com website: http://www.drugs.com/mtm/ampicillin.html
Corleone, J. (2011, August 20). Interactions of cranberry juice & amoxicillin [Interactions of cranberry juice & amoxicillin]. Retrieved October 18, 2011, from Livestrong website: http://www.livestrong.com/article/522171-interactionsof-cranberry-juice-amoxicillin/
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Cranberry Juice [Effects on E. coli /urinary infections]. (2007). Retrieved October 8, 2011, from www.CranberryJuice.eu website: http://www.cranberryjuice.eu/
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Dalbey, M. (2001, December 26). E. coli hazard [The use of E. coli in laboratories and corresponding strains]. Retrieved October 18, 2011, from Biosafety website: http://bio.research.ucsc.edu/Safety/colihaz.html
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Dellis, S., Dr. (2009). Topic I: Handling microorganisms. In Safety procedures in the molecular biology lab (pp. 1-2) [Brochure]. Retrieved from http://delliss.people.cofc.edu/virtuallabbook/LabReadings/SafetyHandout.pdf
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Dixon, A. (2010, September 25). The history of pomegranate juice & its nutritional value [History of pomegranate juice]. Retrieved October 18, 2011, from Livestrong website: http://www.livestrong.com/article/258877-the-history-ofpomegranate-juice-its-nutritional-value/
Dorsey, M. (2006, September 10). Compounds in cranberry juice show promise as alternatives to antibiotics [The ability of cranberries to transform E. coli bacteria]. Retrieved October 18, 2011, from Natural News website:
http://www.naturalnews.com/020947_bacteria_juice_cranberry.html
Kiani, J., & Imam, S. Z. (2007, October 30). Medicinal importance of grapefruit juice and its interaction with various drugs. Nutrition Journal. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147024/?tool=pmcentrez
Macrae, F. (2008, August 19). Drinking fruit juice ‘may stop medication working’ [Fruit juice hurting the effects of antibiotics]. Retrieved October 18, 2011, from Mail Online website: http://www.ehow.com/info_7867586_foods-avoidantibiotics.html
Mayo Clinic Staff. (2010, November 6). Grapefruit juice: Beware of dangerous medication interactons [Grapefruit interactions with medication]. Retrieved October 18, 2011, from Mayo Clinic website:
http://www.mayoclinic.com/health/food-and-nutrition/AN00413
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OceanSpray. (2011). Grapefruit history [History of grapefruit]. Retrieved October 18, 2011, from OceanSpray website: http://www.oceanspray.com/Who-We-Are/Heritage/Grapefruit-History.aspx
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Skipor, A., Ph.D. (2011, October). E. coli safety [Safety precautions while working with E. coli]. Retrieved October 18, 2011, from Newton website: http://www.newton.dep.anl.gov/askasci/mole00/mole00691.htm
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Stevens, E. (2010). History of orange juice [The history of orange juice]. Retrieved October 18, 2011, from The Best of Raw Foods website: http://www.thebestofrawfood.com/history-of-orange-juice.html
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Thomson Reuters Micromedex. (2011). Ampicillin Sodium [Consumer information of Ampicillin sodium]. Retrieved October 18, 2011, from Drugs.com website: http://www.drugs.com/cons/ampicillin-sodium.html
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TOKU-E. (n.d.). Ampicillin - MATERIAL SAFETY DATA SHEET [Safety information of Ampicillin]. Retrieved October 18, 2011, from Toku- E website: http://www.toku-e.com/Upload/products/msds/20110316001358.pdf
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United Press International. (2008, February 29). Pomegranate juice the king of antioxidants [Antioxidants in pomegranates]. Retrieved October 18, 2011, from United Press International website:
http://www.upi.com/Health_News/2008/02/29/Pomegranate-juice-the-king-of-antioxidants/UPI-12261204324911/
Virgo Publishing. (2007, May 29). Study reveals pomegranate juice effective in fighting bacteria [Effectiveness of pomegranate juice in killing bacteia]. Retrieved October 18, 2011, from Infection Control Today website:
http://www.infectioncontroltoday.com/news/2007/05/study-reveals-pomegranate-juice-effective-in-figh.aspx
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Wasenaar, T., Dr. (2011, October). Ampicillin and bacteria [How ampicillin destroys E. coli]. Retrieved October 18, 2011, from Newton website: http://www.newton.dep.anl.gov/askasci/bio99/bio99595.htm
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Wassenaar, T. M., Dr. (2009, January 6). Antibiotics [Overview of antibiotics]. Retrieved October 18, 2011, from The Virtual Museum of Bacteria website: http://www.bacteriamuseum.org/cms/How-We-Fight-Bacteria/antibiotics.html
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Zmuida, C. (2011, January 11). Foods to avoid while on antibiotics [Types of food to avoid while taking antibiotics]. Retrieved October 18, 2011, from e-How Health website: http://www.ehow.com/info_7867586_foods-avoidantibiotics.htm