Transcript Presentation - Harlem Children Society

DEREK KENYENSO
MENTOR:
DR. JAYA SATAGOPAN
HOSPITAL:
MSKCC
DEPARTMENT:
EPIDEMIOLOGY/BIOSTTISTICS
INTRODUCTION
The objective of this project is to
identify genes that have been
differently expressed between recurrent
and non-recurrent prostate cancer
tumors
and to use different ways of calculating
gene expressions.
BACKGROUND ON
PROSTATE CANCER
• What is prostate cancer?
Prostate cancer is the abnormal growth of cell
(tumors) in the tissues of the prostate gland “ the
Prostate gland is a small walnut shaped organ
that lies just below a mans bladder”. Figure below
represents a normal prostate gland.
PROSTATE TUMORS
• This figures shows when there is a prostate tumor in
your prostate gland. After this stage you go to the
doctor to have the tumor removed, after it is removed
then our work comes in.
OUR WORK
• The main question we are trying to
answer is the factors that influence
cancer recurrences.
• To answer this question, we rely on the methods of
Biostatistics.
• What is Statistics? Statistics explores the
collection, organization, analysis, and
interpretation of numerical data. When the focus of
statistics is on biological or medical science it is
called biostatistics.
GENE EXPRESSION DATA
• In this project we are going to compare gene
expressions in recurrent and non-recurrent
patients, using two computer programs: Microsoft
Excel and the R Statistical Package. We will use
the t-test statistic to compare the gene
expressions. The data available to us consists of
the expression levels of more than 44,000 genes
from each of 37 recurrent patients and 42 nonrecurrent patients.
GENE EXPRESSION
DATA CONTINUES
• Gene expressions are obtained using the
HU133A and the HU133B affymetrix
oligonucleotide arrays (www.affymetrix..com ).
Each array consists of approximately 22,000
genes or probes. The methods for processing
patients RNA and obtaining data from
oligonucleotide arrays are described in the
summer project report of Ms. Christina Wassel
(2001).
Recurrent/Non-Recurrence
Patients
Recurrent Prostate Cancer Patients are patients
that have been diagnosed with prostate cancer
and after the tumor is removed, there are
occurrences of the cancer after some time.
Non-Recurrent Prostate Cancer Patients are
patients that after the tumor is removed, there
are no occurrences of the cancer.
Acknowledgements
• Dr. Sats
• Dr. Jaya Satagopan.
• Harlem children society
• MSKCC
• And the Department of Epidemiology and Biostatistics
at MSKCC.