Transcript Diapositiva 1

University of Messina - Italy
Department of Human Pathology
Risk evaluation in breast cancer:
a preliminary study
of pre-metastatic niche
in 252 lymphnodes
A.Ieni, A. Simone, G. Neri, V. Barresi, G. Giuffrè, B. Adamo, N. Caristi,
F. Colonnese, M.A. Gioffrè Florio, V. Adamo, G. Tuccari
National Congress SIAPEC – IAP Florence 7-9 September
Background
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
Bone marrow-derived hematopoietic
progenitor cells (HPCs) seem to
participate in early spread of cancer
cells through the formation of a peculiar
and highly organized micro-environment
for distant tumour growth called
“Pre-Metastatic Niche (PMN)”.
The identification of such HPCs in PMNs
is emerging as a key step in the
assessement of risk for metastases.
Materials
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
In order to identify HPCs of the
premetastatic niche in breast cancer, we
have analyzed 252 lymphnodes(17 of which
pN1a), obtained from 25 patients (mean
age 58.6; age range 47-79 yrs), surgically
treated in the period 1998-2000 for ductal
invasive carcinomas.
The mean follow-up was 84.2 mo. (range
36–136).
Methods
Samples have been fixed in 10% neutral
buffered formalin for 12-72 hrs and included in
paraffin at 56 °C; 4 m thick serial sections were
pre-treated in microwave owen in 10 mM citric
acid, ph 6.0 and incubated overnight with the
folllowing poly/monoclonal antisera:
a. VEGF-R1 (Santa Cruz Biothechnology 1:400)
b. CD 133 (Abgent 1:80)
c. CD 117 (DAKO 1:500)
d. CD-34 (DAKO 1:50)

Results
VEGF-R1
Results
CD 133
Results
CD 117
Results
CD 34
Results
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In lymphnodes, clusters of immunoreactive cells
were always evident with CD 117 and CD 34.
A different amount of lymphnode immunopositivity
was found with VEGR-R1 and CD 133 antibodies in
relation to different metastatic sites. In particular,
breast cancer cases with a follow-up history
characterized by a metastatic spread in lungs, liver
and
central
nervous
system
were
more
immunoreactive than those with bone localization.
Seventeen lymphnodes classified as pN1a showed
no immunoreactivity.
Conclusions


The clinical significance of “node negative”
breast carcinomas needs to be further
investigated, since the identification of HPCs
able to predict possible, imminent or future,
spread to other sites becomes mandatory.
The immunomorphological research for
PMNs in the lymphnode microenvironment
could have significant clinical implications for
the breast cancer treatment.