Transcript - Global Farm Platform

Anees C*, and Majumdar A C
Reproductive Physiology and ETT Laboratory
Division of Physiology and Climatology
Indian Veterinary Research Institute, Bareilly,
Uttar Pradesh, India, 243122
•Corresponding Author: [email protected]
Population Explosion
Global warming
2050: Projections (2005-07 baseline)
• Global consumption of meat- 76% Increase
• Global consumption of dairy- 65% increase
(Rob Bailey Energy, Environment and Resources - Dec 2014)
• Shall we multiply the number of Animals ???
Nitrous oxide
CO2
Methane
Hostile Climate for Production (Hot and Humid –
especially in Kerala, Gujarat, Orissa and West Bengal)
Few well adapted animals ( Both high producing as
well as disease resistant )
Propagation of these well adapted animals by
Totipotent Stem cell cloning / Culture - Concept paper
(Majumdar 2005)
Oocyte and embryo like structures including blastocyst
like structure developed from pluripotent stem cell
(Majumdar et al 2002)
Mouse embryonic stem cells formed oocytes, embryo
and blastocyst like structure
(Hubner et al 2003, Science)
1. To develop embryo like structures from
Totipotent stem cells (Goat Model)
2. To study the further development of
embryo like structure if any, in recipient
• In vitro fertilization
(As per standard procedure in the laboratory Anees 2007)
• Isolation of totipotent stem cells
(Standard protocol , Swain et al 2002)
• Culturing of these cells to develop embryo like
structures
The Concept of Superior Quality
Animal multiplication
Blastomeres
In vitro
Embryos
Embryo like structure
Superior Quality Animal
ELS has the potential to become an animal
 Selection of good quality ELS from culture
 Surgical Transfer of these ‘embryos’ to
synchronous recipient
 Evaluation of development
Immature oocytes
Mature oocytes
8-16 cell embryos
Embryo Like Structures (ELS)
Blastocyst like structures
Cells hatching out of ELS
Hubner et al 2003
Relative expression of development related genes in Embryo like structures (24
nos) in comparison to 24 number of IVF derived 8 cell stage embryos and 3 number
of IVF derived 8 cell stage embryos.
6.00
5.00
fold changes
4.00
8 cell Oct-04
8 cell Nanog
3.00
8 cell Sox 2
8 cell, 24 Oct-04
2.00
8 cell, 24 Nanog
8 cell, 24 Sox 2
1.00
0.00
TELS
Biol.incu
culture condition
PELS
• ANEES cardiomyocites KSC.avi
Characterized
using marker
gene NKx 2.5
• Two out of the three recipients returned to heat
21days post estrus
• One recipient returned to next cycle 28 days post
estrus
• Further investigations are on
 Embryo Like Structures were developed from
totipotent stem cell culture
 They had similar but lower gene expression
compared to IVF Embryos
 Further investigation is required to improve the cell
size and to produce live animal from these cells
 Generation of ELS and its development can address
the environmental challenges