Transcript Plant-virus interactions INRA Bordeaux-Aquitaine

Plant-virus interactions
INRA Bordeaux-Aquitaine
IBVM,
INRA Bordeaux-Aquitaine
History of the group
• Fruit tree viruses: started in the 60’s
• In the 90’s: 3 focuses
– Virus identification, detection and populations
– Virus resistance using transgenic plants
– Natural virus resistance: host-virus interactions
• This last item was re-focused
– Nepoviruses decreased
– Lettuce mosaic virus started and took over in 1999
Plant-potyvirus interactions
• Identify virus determinants
– Necrosis in the lettuce cultivar « Trocadéro »: HcPro
– Ability to overcome mo1: central region of LMV genome
• mo1 encodes eIF4E
– 2 alleles in 2 types of genotypes in crop lettuce
– 1 more haplotype in crop lettuce
– 1 more haplotype in weed lettuce
• How does it work?
– Quality / structural aspects of the interactions
• Yeast two-hybrid, ELISA, spectrofluorescence
– Other partners?
• LMV HcPro inhibits the proteasome’s RNase
Plant-potyvirus interactions: genetics
• Genetic approach implemented in 2 hosts:
– Apricot
– Arabidopsis
• In Arabidopsis:
– Screen ecotype collections with virus collections (PPV, LMV)
– Identify many sources of resistance
• Local – Systemic – Symptoms
• Monogenic – Oligogenic
• Dominant – Recessive
– Begin to clone some of these resistance sources:
• rlm1
• rpv1
 Next needs: genetic ressources to go back to
crop species