Transcript 分 子 病 理 實 驗 室 Molecular Pathology Lab 實驗室主持人

分子病理實驗室
Molecular Pathology Lab
實驗室主持人：戚謹文, 周德盈
共同主持人: 李芬瑤
The development of rational
approaches to the diagnosis and
treatment of cancer is dependent
on the understanding of the
mechanism that underlie the
formation and progression of
tumor
Traditional pathology
Molecular pathology
•Traditional pathology
1. Frozen section
2. Wax embedded tissue
Hematoxylin-eosin stain
Special stain:
silver stain
immunostain: vessel,
estrogen receptors
•Advances in molecular biology
Genetic analyses
Comparative genomic hybridization(CGH)
Fluorescence in situ hybridization (FISH)
Gene expression: transcription
Genomic fingerprinting
cDNA and oligonucleotide arrays
Serial analysis of gene expression
Differential display or representational difference
analysis
Gene expression: translation
2-D gels, HPLC, Mass spectrometry, tissue array,
microdissection and laser microscopy
•Molecular pathology (1)
Human genome project
1. novel genes
2. known genes :
amplification,
mutation,
deletion
•Molecular pathology (2)
Human genome project
Studies of mRNA can determine
the expression profile of a large
number of transcripts.
•Molecular pathology (3)
Human genome project
Proteomic methodologies can
identify quantitative and
qualitative protein changes in
tumors, and to examine
posttranslational modifications
such as phosphorylation of proteins.
Loss of annexin 1 correlates with early onset
of tumorigenesis in esophageal and prostate
carcinoma. Paweletz et al. Cancer Res 60:6293, 2000
•References:
1. Going and Gusterson Molecular pathology and
future development. Eur J Cancer, 35:1895-1904,
1999.
2. Sirivatanauksorn et al. Laser-assisted
microdissection: applications in molecular biology.
J Pathology 189: 150-154, 1999.