Transcript exam cls 453

Genetics practical exam final
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Name
Number
A)
X XY
B)
Section 1:
Q1: In karyotpes A and B mention the abnormalities seen and the name of the disease in karyotype A
and if known the name of the disease in B
Q2:Describe the kind of disorders that can be diagnosed by cytogentic means
Q3: Why are cells cultured before cytogentic analysis?
Q4: Why do we add a hypotonic solution to cultured cells prior to cytogentic analysis?
Q5: Why are cells dropped from a distance on glass slides in cytogentics?
Section 2:
Q1: Design an appropriate PCR program to amplify a genomic fragment
1205bp long using the following primers
Primer 1 5'-GCTACGGAAATCATGACTAC-3'
Primer 2 5'-CGCTATCTACGGCATTATCC-3'
Tm 58
Tm 53
Q2: In your own words, what could result form the establishment of strong
dimer structures between a primer pair?
Q3: Why do we add taq polymerase to the PCR?
Q4: What could result if we accidentally did not add one of the primers to the
PCR?
Q5: In the following sequence design DNA primers to amplify the area in bold
AAGAATCATTCTGTCACCTGACCCCAATTCCTTGACCTTGTTACAGAAAAGGTTTATGTA
ATTATTGCAAGACATCTACATATCCCTACATAGATGGAAAACAATAATACATCTTATACA
TACATATACTATTCATTTTTCACATGTAAGTAGGTGTTGTAATCAATATGACACAAATGG
TTTTAATAACATTAAATTTATTTGACAGTATGTATTTTCTTATTCAATATGTAAATATAG
GCAAGAGATTTTACTACTATCATGAGACATTTATGAATTCTTAGAAAGGAATTCTCACTT
TTTAAAAACAATTGGGAATTGCTAATGTAAATAATGATGGTTCCTTGTGGTCAGCAAAAT
GGAAAGAAACATGGAAGGGGTCGGAAATATGAAGGCACTTACACACACACACACACACGC
ACACAAAAGCTATCAATTTCTGTCAGAAAATTGGCTGACGCATCCTTGCTTTACCTTGGA
GCTATGGGAGGTAGCTCTTGATTTAGCTGCTTAATTTAGCATGGGTGTTAAAGAACCCTG
TGTCAGCAAACTCACAGGAAGAACTTGTCATTTCTATTGGTATTCTGTCTAAGCTTCAAA
Section 3:
Q1: The following plasmids (A, B and C) have bean digested using EcoRI and BamHI (the restriction
sites are indicated on the plasmids) using your pin draw the predicted bands in gel picture below with
consideration to size
(B)
(A)
(C)
BamHI
BamHI
3kb
2.5kb
4kb
BamHI
5kb
HindIII
11kb
10.5kb
BamHI
1kb
2.5kb
BamHI
0.5kb
1kb
6kb
BamHI
EcoRI
(A)
12kb
10kb
9kb
8kb
6kb
4kb
3kb
2kb
1kb
0.5kb
0.4kb
0.3kb
(B)
(C)
6kb
EcoRI
Q2: Match restriction ends from column A to there corresponding end in column B
A)
B)
CTGATGCC
GACTA
ATTGGCGC
CCGCG
CTCGCT
GAGCGA
CATTA
CGCGTAAT
GGTAC
CCATGTAA
CGTC
CGGGCAG
GCTGGCG
CGAC
ATATAG
TATATC
Section 4:
Q1:What is the figure above called?
Q2: How many genes are present in the figure?
Q3: How may interons and exons are in each gene?