Transcript lactase_and_evol2 - BioQUEST Curriculum Consortium

Lactase genes and evolution
Points of concern
• Lactase and curriculum
– General Biology course
– General Microbiology
– Genetics
• What is the problem?
– Students misconceptions
Data set
Mammals
10
Bacterial
Lactase 40
Small Subunit 6
Large subunit 9
Invertebrates
4
86
Fungi
6
Archaebacteria
4
Plants
7
resources
• See images.ppt and .doc files for visual
database of organisms from which
sequences were obtained
• Sequence files (.doc) are organized by
type of organism and lactase subunit
Sample Questions: Level 1
• Is the bacterial lactase gene similar to the
human lactase gene?
• Is this gene exclusive to eukaryotic or to
prokaryotic organisms?
• How is this gene distributed across the
organisms?
Sample Questions: Level 2
• How does the bacterial lactase gene
compare to archaebacteria?
• What evolution scheme can be inferred?
• Describe the evolutionary relationships
within and among the different groups of
organisms.
Plant data
Unrooted tree for plant data
Splt Decomposition (ESTEEM module) to determine relationships
between four plants based on lactase sequence
http://www.ihop-net.org/UniPub/iHOP/
References
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Genoscope Centre national de séquençage
http://www.genoscope.cns.fr/externe/English/corps_anglais.html
BRENDA: The Comprehensive Enzyme Information System
http://www.brenda.uni-koeln.de/index.php4
Genomics: GLT, Systems Biology for Energy and Environment
http://genomicsgtl.energy.gov/links/archaea.shtml
Genomes News Network
http://www.genomenewsnetwork.org/categories/index/genome.php?s=0&n=
15
Doe Joint Genome Institute http://www.jgi.doe.gov/
Fungal Cell Biology Group http://129.215.156.68/index.html
BaNG: Nematodes and Neglected Genomics
http://zeldia.cap.ed.ac.uk/index.shtml
iHOP- Information Hyperlinked over proteins http://www.ihopnet.org/UniPub/iHOP/
European Molecular Biology Laboratory, European Bioinformatics Institute
(EMBL-EBI) http://www.ebi.ac.uk/clustalw/
National Center for Biotechnology Information http://www.ncbi.nlm.nih.gov/
Biology Workbench http://workbench.sdsc.edu/