Transcript Nov.12th - University of Maine System

Day
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Th
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Th
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Th
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Date
Reading Topic
10/15
no class – October Vacation
10/17
More plant development
10/22
Ch. 8
Mechanisms of morphogenesis
10/24
More morphogenesis
10/29
Ch. 10
Cell differentiation
10/31 Happy Halloween!
Stem cells
11/5
quiz on Flowering video handout
Ch. 11
Organogenesis
Th
T
Th
11/7
11/12
11/14
T
11/19
Th
More Organogenesis
Ch. 12
Development of the nervous system
EXAM 2 Chapters 2, 7, 8, 10
Drosophila, plants, cell biology, differentiation
11/21
Ch. 9
The saga of the germ cell
T
11/26
More on reproduction
term paper is due at the beginning of class on Tuesday, 11/26
Th
11/28
Your
no class – Thanksgiving Break
Also remember there are two, short writing assignments, each worth 20 points.
Pick any topic in developmental biology (except your term paper topic) and learn more about it. Then
write a two page paper and include all the sources you used (those can include any sources including web
sites, newspapers, TV, etc). These are due anytime.
In-Class Assignment:
Draw a human, neural crest cell with lamellopodia and show the direction of
movement as it crawls over the extra cellular matrix (ECM). Include labels or a
key for your drawing.
Show the correct position of the transmembrane protein integrins (the cell
adhesion molecule for this example) within membranes throughout your
drawing.
Show three parts of the lamellipodium:
1) Actin microfilaments polymerizing and growing new tips of
the
lamellipodium
2) microfilaments failing to make contact and depolymerizing
3) making contact between the integrins and the ECM and
microfilaments
contracting as actin and myosin slide past each other
Show integrins grabbing hold of the ECM in the front, leading edge of the cell
and letting go at the back of the cell.
Show the exocytosis and endocystosis pathway moving integrins from the
back of the cell to the front.
Show microtubules with dynein and kinesin protein motors attached to them
and also attached to vesicles with integrins. Include arrows to show the
vesicles are transporting integrins from the back to front, leading edge of the
cell.
Ch. 10: Cell Differentiation, Stem Cells
10.1 differentiation
10.23 neural crest derivatives
Fate
Specify
Determined (committed)
Differentiate = the cell becomes the final cell type
differentiation is irreversible
Fig. 10.19
“master regulators” like MyoD are transcription factors that
can cause cells to become determined when the gene is expressed.
Fig. 10.19
Fig. 10.3 transcription model
Fig. 10.2
The story of creating Induced Pluripotent Stem Cells (IPSC)
2006 – present. (2012 Nobel Prize in Medicine)
Fig. 10.28
The story of creating Induced Pluripotent Stem Cells (IPSC)
2006 – present. (2012 Nobel Prize in Medicine)
Four pivotal genes (each encodes a transcription
factor) :
Oct3/4
Sox2
Klf4
c-Myc
BOX 10C
BOX 10C