Transcript lin-14(sd)

Scanning em of C. elegans
Crawl movie
Fly-in movie
Male, scanning
em
Male tail scanning em
Rene Mating Movie
Embryonic cell lineage
D lineage
ABala lineage
AIML lineage
AIML neuron
AIML circuit
Postembryonic blast cells
Z1Z2 Z3 Z4
Adult hermaphrodite anatomy
C. elegans typical cross section
Seam cell postembryonic lineages in the male
wild type
lin-4(e912)
reiterations
wild type
lin-14(sd) repeats S1
lin-14(lf) skips S1
lin-4(e912)
lin-14(sd)
lin-4(lf) and lin-14(gf) have the same phenotype: they repeat S1.
LIN-4S is complementary to sequences in the 3’UTR of lin14.
Human miRNA genes
Symmetric neuron phenotype of lsy-6(-)
A regulatory pathway leading to neuron asymmetry
involving two micro RNA’s
Worms Eating Bacteria Expressing dsRNA to
a GFP Reporter Gene Activate RNAi
wild type worms
RNAi-defective mutants
from Mello and Conte,
Nature 431, 338-342.
GFP lost in embryos
GFP lost in the gut
RNAi in Plants
1928 report of development
of viral (tobacco ringspot virus)
resistance in tobacco, now
known to be due to endogenous
RNAi.
RNAi/miRNA Pathways
from Meister and Tuschl, Nature
431, 343-349, 2004.
The Structure of Argonaute (Slicer)
Song et al., Science
305, 1434-1437, 2004.