Transcript Dormancy of cells and organisms -strategies for survival and

Dormancy of cells and organisms strategies for survival and
preservation
NEST - # 012674
2005-2008
Acronym: Sleeping Beauty
Partners
• Esther Lubzens (NIO) Ora Hadas, Assaf Sukenik (KLL) -IOLR –
P1
• Joan Cerdà, IRTA, Barcelona, Spain – P2
• Stefan Hohmann, Goteborg University, Sweden – P3
• Melody Clark and Roger Worland, BAS, Cambridge, UK – P4
• Richard Reinhardt, Max Planck Institute –Molecular Genetics,
Berlin-Dahlem, Germany – P5
• Jens Nielson, Danish Technical University, Lyngby, Denmark - P6
Objectives
Learning from nature to develop new
concepts for cell preservation based on
mechanisms with which model organisms
tolerate desiccation and/or remain
metabolically inactive for long periods
Goals
• Reveal strategies employed by different organisms that
facilitate their long term survival in a dormant form
• Widening the basis of our knowledge on the molecular details of
strategies employed by 5 model organisms
• Resolve whether these strategies can be used to develop
preservation (“ reversible dormancy ”) approaches at ambient
temperatures for different cells and organisms
* Emphasis on understanding processes that control the
establishment and maintenance of dormant stages and the exit
from dormancy
Specific goals
WP 1 & 2 Discover the genes, proteins and compounds
that are associated with the formation of dormant
forms and exit from it in:
Cyanobacteria
Yeast
Rotifers
WP 3. Discerning the processes associated with
desiccation as a survival strategy by global gene
expression, proteome and metabolome profiling
Arctic springtail
killifish embryos
Specific Goals - Continued
WP 4. Confirming the role of specific genes in tolerance
to desiccation or dormancy by genetic analyses and
genetic genetic engineering experiments
Yeast
Killifish
Rotifers (genetically selected strains)
WP 5. Discovering common and divergent strategies
through synthesis of the genomic, proteomic and
metabolomic data of model organisms and of data
from other organisms, including plants – in silico
models
Specific Goals - Continued
• WP 6. Publicity to stir and encourage new research
direction
Scientific publications, scientific meetings
Workshop
Book
• Tools: High through approaches; genomics,
proteomics, metabolomics and bioinformatics
Partners
• Ora Hadas, Assaf Sukenik (IOLR)- Cyanobacteria
• Joan Cerdà, IRTA, Barcelona, Spain - Killifish
• Stefan Hohmann, Goteborg University, Sweden - yeast
• Melody Clark & Roger Worland, BAS, UK – Arctic sprintail
• Richard Reinhardt, Max Planck Institute –Molecular Genetics,
Berlin-Dahlem, Germany – Genomic and proteomic platform
• Jens Nielson, Danish Technical University, Lyngby, Denmark metabolomic platform
WP Number
Title
Leading partner & Starting
Participating
month
partners
WP0
Management
Esther Lubzens
WP1
Establishment of
dormant stages
Esther Lubzens &
M0
Ora Hadas- P3, P5,
P6
WP2
Arousal from
dormancy
Stefan Hohmann –
P1, P5, P6
M0
WP3
Desiccation and
revival
Joan Cerdà – P4,
P5, P6
M0
WP4
Engineering
/genetic testing
Joan Cerdà – P1,
P3, P5, P6
M15
WP5
Common and
divergent
pathways
Stefan Hohmann &
Melody Clark – P1,
P2, P5, P6
M0
WP6
Dissemination
Esther Lubzens –P2, M12
P3, P4, P5, P6
M0
Pert Chart for SLEEPING BEAUTY
WP1
Dormant stages
P1, P3, P5
WP2
Germination and arousal
from dormant stages
P1, P3, P5
WP3
Resistance to desiccation
P2, P4, P5
WP1
Dormant stages
P1, P3, P5, P6
WP2
Germination and arousal
from dormant stages
WP4
P1, P3, P5, P6
Engineering /genetic
testing strategies
WP3
P1, P2, P3
Resistance to desiccation
P2, P4, P5, P6
WP5
Identification of common pathways for dormant stages,
revival/germination and resistance to desiccation
P1-P6
WP6
Participation in scientific meetings, organization of a
workshop and publication of a book on dormancy and
resistance to dessication
P1-P6
Towards novel concepts and methods for
cell preservation