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α-ketoglutarate signals
N-deprivation in an association
between Nostoc punctiforme
and Anthocerus punctatus
Jeff Elhai
Background
Introduction
The world population is the total number of living humans on Earth. As of
today, it is estimated to number 7.055 billion by the United States Census
Bureau (USCB).[1] The USCB estimates that the world population exceeded
7 billion on March 12, 2012.[2]
To feed an evergrowing population, it is necessary to increase the
availability of food. Green Revolution refers to a series of research,
development, and technology transfer initiatives, occurring between the
1940s and the late 1970s, that increased agriculture production around the
world, beginning most markedly in the late 1960s.
Norman Borlaug is considered the Father of the Green Revolution.
With advances in molecular genetics, the mutant genes responsible for
Arabidopsis thaliana genes (GA 20-oxidase,[17] ga1,[18] ga1-3[19]), wheat
reduced-height genes (Rht)[20] and a rice semidwarf gene (sd1)[21] were
cloned.
Green revolution plants are able to make use of more N fertilizer.
Additional
Background
Introduction
Nostoc punctiforme forms symbioses with many plants. It is a
heterocystous cyanobacterium that fixes nitrogen in semiregularly spaced
cells.
An experiment
Nostoc passes fixed nitrogen to the plant
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Materials and Methods
GltI was amplified from the genomic DNA by PCR and
subcloned into pRSETB (Invitrogen). Truncation
combinations were amplified with Phusion polymerase
(NEB) and purified with 96-well PCR cleanup
cartridges (Qiagen).
Results
These are the kind of results I expect to get.
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More results
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
Discussion
World hunger is one of the gravest threats facing our
world today. Every year 15 million children die of
hunger. If this experiment helps even a small fraction
of them it will be worth it.
References
Any Questions?