Transcript Induced pluripotent stem (iPS) cells

Induced pluripotent stem (iPS)
cells
Patient-Specific Pluripotent Stem Cells
Nuclear reprogramming to a pluripotent state by three approaches
Shinya Yamanaka & Helen M. Blau
NATURE|Vol 465|10 June 2010|doi:10.1038/nature09229
The fusion experiments by Tada, Surani and colleagues
clearly showed that ES cells and embryonic germ cells
contain factors that can induce reprogramming and pluripotency
in somatic cells*.
Before 2006, the prevailing view was that nuclear reprogramming
to a pluripotent state is a highly complex process that might entail
the cooperation of up to 100 factors**.
*Tada, M., Tada, T., Lefebvre, L., Barton, S. C. & Surani, M. A.
Embryonic germ cells induce epigenetic reprogramming of somatic nucleus
in hybrid cells. EMBO J. 16, 6510–6520 (1997).
**Nuclear reprogramming to a pluripotent state by three approaches
Shinya Yamanaka & Helen M. Blau
NATURE|Vol 465|10 June 2010|doi:10.1038/nature09229
Nuclear reprogramming to a pluripotent state by three approaches
Shinya Yamanaka & Helen M. Blau
NATURE|Vol 465|10 June 2010|doi:10.1038/nature09229
b-geo cassette (a fusion of the b-galactosidase
and neomycin resistance genes)
24 genes
Oct3/4, Klf4, Sox2, and c-Myc
Morphology: 5/12 survived
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult
Fibroblast Cultures by Defined Factors
Kazutoshi Takahashi and Shinya Yamanaka
Cell 126, 663–676, August 25, 2006
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult
Fibroblast Cultures by Defined Factors
Kazutoshi Takahashi and Shinya Yamanaka
Cell 126, 663–676, August 25, 2006
Gene-Expression Profiles of iPS Cells
Induction of Pluripotent Stem Cells from Mouse Embryonic and
Adult Fibroblast Cultures by Defined Factors
Kazutoshi Takahashi and Shinya Yamanaka
Cell 126, 663–676, August 25, 2006
they are different with regards to gene
expression and DNA methylation
patterns, and fail to produce adult
chimaeras.
Pluripotency of iPS Cells Derived from MEFs
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
Kazutoshi Takahashi and Shinya Yamanaka
Cell 126, 663–676, August 25, 2006
Generation of germline-competent induced pluripotent stem cells
Keisuke Okita, Tomoko Ichisaka & Shinya Yamanaka
Vol 448| 19 July 2007| doi:10.1038/nature05934
The generation of iPS cells from adult human dermal fibroblasts
with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Human iPS cells were similar to human embryonic stem (ES) cells
in morphology, proliferation, surface antigens, gene expression,
epigenetic status of pluripotent cell-specific genes, and telomerase
activity. Furthermore, these cells could differentiate into cell types
of the three germ layers in vitro and in teratomas.
These findings demonstrate that iPS cells can be generated from
adult human fibroblasts.
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts
by Defined Factors
Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki, Megumi Narita, Tomoko
Ichisaka, Kiichiro Tomoda, and Shinya Yamanaka1,2
Up to 10% of transduced mouse embryonic fibroblasts lacking
p53 became iPS cells, even without the Myc retrovirus. The p53
deletion also promoted the induction of integration-free mouse
iPS cells with plasmid transfection.
Furthermore, in the p53-null background, iPS cells were
generated from terminally differentiated T lymphocytes. The
suppression of p53 also increased the efficiency of humaniPS
cell generation.
Suppression of induced pluripotent stem cell generation by the p53–p21 pathway
Hyenjong Hong, Kazutoshi Takahashi, Tomoko Ichisaka, Takashi Aoi, Osami
Kanagawa, Masato Nakagawa, Keisuke Okita & Shinya Yamanaka
Vol 460|27 August 2009| doi:10.1038/nature08235
Nuclear reprogramming to a pluripotent state by three approaches
Shinya Yamanaka & Helen M. Blau
NATURE|Vol 465|10 June 2010|doi:10.1038/nature09229
Timeline of discoveries in nuclear reprogramming.
Nuclear reprogramming to a pluripotent state by three approaches
Shinya Yamanaka & Helen M. Blau
NATURE|Vol 465|10 June 2010|doi:10.1038/nature09229
Shinya Yamanaka, Professor of Kyoto University,
won the Albert Lasker Basic Medical Research Award in 2009.
Often referred to as “America’s Nobel,”
the Lasker Award is one of the most prestigious scientific awards.