Transcript how environmental factors influence transcription, gulf coast 2013

Teachable Tidbit:
Gene Expression
Venu Cheriyath, TAMU Commerce
Jeff Kopachena, TAMU Commerce
Christie Landry, Nicholls State University
Michelle Thiaville, Nicholls State University
FACILITATORS:
Amy Briggs
Elizabeth Eich
LEARNING GOAL
• Students will know how environmental factors
influence transcription.
LEARNING OUTCOME
• Construct a diagram of how an environmental
factor can result in changes in gene expression
that changes cellular function
What causes altitude sickness?
How does your body
recover from altitude
sickness?
ACTIVITY 1:
• Gather into groups
• Collect an envelope containing word strips
• Arrange the molecular events in the order
that they occur in the cell
• You have 5 minutes…GO!
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Review the strip sequence of the
groups having the same color strips
as you.
• DO YOUR SEQUENCES MATCH?
Activity 2:
• Pair with a group that has a different color strip
sequence than yours.
• Compare your strip sequences.
– Which color group comes first?
Strip Sequence Order
1.
2.
3.
4.
5.
6.
7.
Low oxygen environment is encountered
Body detects hypoxic condition, and
kidney interstitial fibroblasts initiate
response to low oxygen tension
Stabilization of Hypoxia Inducible Factor
(HIF) occurs, and it is translocated into
the nucleus
Hypoxia inducible factor (HIF) binds to
Erythropoietin (EPO) gene promoter
Erythropoietin gene is transcribed
Erythropoietin mRNA is translated
Erythropoietin protein is exported from
the cell
1.
2.
3.
4.
5.
6.
7.
8.
Erythropoietin (EPO) binds to
Erythropoietin Receptor (EPO-R) on
Erythrocyte Progenitor Cells
Erythropoietin (EPO) binding induces
conformational changes to occur in
Erythropoietin Receptor (EPO-R)
Activation of Janus Kinase (JAK) occurs
Janus Kinase (JAK) activates its
substrate, STAT5, a transcription factor
Activated STAT5 translocates into the
nucleus
STAT5 binds to promoter elements of
proliferation-inducing genes
Expression of proliferation-inducing
genes
Increased production of Red Blood Cells
Activity 2:
• In your combined color groups:
– Based on the projected template, draw the sequence
of events represented in both strip sequences
• Post your finished diagram on the wall or board.
• You have 5 minutes…GO (again)!!
EPO
End
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HOMEWORK:
• Using the following potential environmental
exposures, draw a picture showing how gene
transcription would be initiated/affected by the
exposure.
• Be prepared to present your drawing in the next
class period.
 Steroid Mimetics (Group 1 & 2)
 Pesticides (Group 3 & 4)
 Photoperiod (Group 5 & 6)
 Nutrient deficiency (Group 7 & 8)
 Inflammation/Injury (Group 9 & 10)