Transcript Reading Primary Literature

Reading Primary Literature
in Intro Bio
Anne Casper
Eastern Michigan University
Lab Report Assignments for our
Intro Bio students
Writing Assessment Rubric
Relevance
of sources
3
Sources are used
that address the
argument(s) or
idea(s) from
multiple angles.
2
Sources
directly
support the
argument(s)
or idea(s)
1
Sources are
tangential to
argument(s) or
idea(s)
0
No sources, or
sources are
listed in works
cited but not
used in body
of text.
Writing Assessment Rubric
3
Selection of Use of BOTH
Resources* primary and
secondary
literature, with
good reliance on
primary
literature. No
reliance on
tertiary literature.
2
1
Use of
Some reliance on
primary or
tertiary
secondary
literature, but
literature. No also use of either
reliance on
primary or
tertiary
secondary
literature.
literature.
0
Complete
reliance on
tertiary
literature
and/or use of
sources
indicates
confusion and
lack of
understanding.
* Primary literature: peer-reviewed publications of original research in scholarly journals
* Secondary literature: peer-reviewed literature reviews published in scholarly journals
* Tertiary literature: text books, internet sites, articles written for a general audience etc.
Writing Assessment Rubric
3
Integration Effectively
of sources integrates sources
into their
argument i.e.,
sources are not
used sequentially
but instead build
around ideas
2
Sources are
generally
sequential,
but student
uses more
than 1-2
sentences
from the
source.
1
Only 1-2
sentences from
source are used
to support the
argument.
(Student does
not appear to
understand
source.)
0
No sources, or
sources are
used
inappropriatel
y, or sources
are listed in
works cited
but not used
in body of text.
Writing Assessment Rubric
3
2
1
Organization Logical
There is a
Organization or
organization of the logical
progression could
work, plus
progression or improve.
outstanding
organization to
transitions and
the work.
clear linking of the
relationships
between sections.
0
Progression or
organization
not logical or
clear.
3.50
3.00
2.50
2.00
BIO 110
BIO 120
1.50
BIO 306, 311W
1.00
0.50
0.00
Relev. of sources
Selection of
sources
Integration of
sources
Organization
How are we introducing our students
to the primary literature?
My goals for reading primary lit
in Intro Bio I
1. Reinforce concepts from lecture and lab activities
2. Demonstrate how concepts from class are applied by
research scientists
3. Increase students’ awareness of how research is conducted
4. Increase students’ ability to read and understand primary
literature
5. Increase students’ ability to integrate information from
primary literature in their lab reports
Start with
“How to Read a Scientific Paper”
http://www.lib.purdue.edu/phys/inst/scipaper.html
General Questions
1. Is this article primary scientific literature or secondary scientific
literature? How do you know?
2. At first, it may look like this article does not begin with an abstract.
But, there actually *is* one – where is this article’s abstract?
3. What is the name of the journal in which this article was published?
In what year was the article published?
4. Where is the research laboratory where the first author of this
article works? (Hint: Look at the end of the article to find this
information.)
5. Where did the authors get the money to support the research in this
article?
Questions on the Introduction
8. Two research groups recently reported what causes HPGS.
a. HGPS is caused by mutations in what gene?
b. In what year did these two groups publish their discovery?
c. What journals did these two groups publish their papers in?
Questions on the Introduction
9. On the inner surface of the membrane surrounding the
nucleus, there is a meshwork of intermediate filaments. This
meshwork, called the nuclear lamina, provides structural support to
the nuclear membrane, and is involved in breakdown and reformation
of the nuclear membrane during cell division.
a. What are the three main components of the nuclear
lamina?
b. Lamin A and Lamin C are both encoded by the same gene.
How is it possible for two different proteins to be made from
the same gene?
Questions on the Introduction
10. Based on the results of the two previous papers about HGPS, does
this disease have an autosomal dominant or autosomal recessive
pattern of inheritance?
10. If there have already been two articles published on what causes
HGPS, why did the authors think it was important to publish this
article, since it’s also on the same topic?
Questions on the Results
Look at the “Carrier” sequence results. Why does the carrier
have one peak in the sequencing read that is overlapping
both blue and yellow?
Questions on the Results
The results of AluI digest of PCR products from particular individuals is
shown in Figure 1C. Look carefully at the result for individual IV-5.
Based on this result, what is the genotype of this individual?
Questions for you…
-What are your goals for having Intro Bio students read primary
literature?
-How did you select papers for students to read? (I spent a long
time finding accessible ones….)
-How have you structured lab or class time for students to read the
articles?
- How have you assessed the success of achieving your goals for
students?