Transcript Document

Searching the NCBI
Databases
Search for CFTR[gene] in the Protein
database
What is it?
Limit to genes in human only
CFTR[gene] AND human[orgn]
How many?
Restrict to RefSeq
How many?
RefSeq records can also be filtered using
the Limits tab
Examine the NP_000483 record
How many amino-acids are in the protein?
On which chromosome is this protein-coding gene
located?
Follow the dbSNP link to examine the SNPs
associated with this protein
How many SNPs?
Follow the dbSNP link to examine the SNPs
associated with this protein
Describe mutation in first record. Is it
synonymous?
Use the Taxonomy Browser to examine
fully-sequenced primates genomes
Use the Taxonomy Browser to examine
fully-sequenced prototheria genomes
Sequence Retrieval
Search for human BRCA1 in Protein
database
Select longest isoform
Display the sequences in FASTA format
Save the sequence into a WORD file