Transcript Document

Potato Genome Project
1.Structural Genomics
Generation of a comprehensive genomic resource of Solanaceae
resistance (R) gene sequences of wild potato, tomato and pepper
genomes (the SOLAR database)
2.Functional Genomics
Generation of a comprehensive functional genomic resource
composed of expression profiles of significant points in growth,
development and response to abiotic and biotic stresses, and a set
of functionally defined genes identified through virus-induced gene
silencing to be involved in host response to pathogens
3.Outreach
A training program for high school and college students to teach
applications of bioinformatics and data mining for marker
development for potato breeding for crop improvement
Synteny between Solanaceae Genomes
1 2 3 4 5 6 7 8 9 10 11 12
The Solanaceae share
highly conserved
genomes
Potato
 Chromosome number and
organization
 Genome sequence
1 2 3 4 5 6 7 8 9 10 11 12
Pepper
 Sequence and function of
gene location and
resistance hotspot location
1 2 3 4 5 6 7 8 9 10 11 12
Tomato
Resistance hotspots
Chromosome 5 Resistance Region
Potato
Tomato
GP21
GP179
GP179
R1, phyt3,
Pi1,Nb, Rx2,
Gpa, Grp
GP21
Bs4
S. demissum is an allo-hexaploid
Chromosome #5
5A
R1, phyt3,
Pi1,Nb, Rx2,
Gpa, Grp
5B
5C
S.demissum Chromosome 5 Resistance Region
8~12 BACs per genome
~0.5Mb per genome
A
GP179
R1
SPUD237
GP179
132D05
132D05S
GP21
Haplotype
Sd_Bs4
R1, phyt3, Pi1, Nb,
Rx2, Gpa, Grp
GP179
GP21
132D05
Chromosome #5
R1
Sd_Pr
f
Sd_Pr
f
B
Sd_Bs4
C
Sd_Prf
S.demissum Chromosome 11 Resistance Region
Potato
Pepper
RMcl, Ry, Na,
Sen1,
R3a, R3b, R5,
R6, R7, R8,
R9, R10, phyt7
Tomato
phyt3
I
I2
L,
cmv1,
phyt3
S. demissum is hexaploid
Chromosome #11
11A
RMcl, Ry, Na,
Sen1, N
R3a, R3b, R5,
R6, R7, R8,
R9, R10, phyt7
11B
11C
S.demissum Chromosome 11 Resistance Region
Haplotype
A
B
56F6R
193I9R
Chromosome #11
39E18F
RMcl, Ry, Na,
Sen1,
S.demissum Chromosome 11 Resistance Region
Haplotype
A
B
C
373T
373T
381T
TG105
381T
11~16 BACs
1.5Mb
373T
381T
Chromosome #11
51S
R3a, R3b, R5, R6, R7,
R8, R9, R10, phyt7
S.demissum Chromosome 11 Resistance Region
R3a, R3b, R5, R6, R7,
R8, R9, R10, phyt7
Haplotype
A
B
C
51S
51S
51S
373T
GP185
373T
12~16 BACs
~1.5Mb
373T
381T
Chromosome #11
Distance tree for all I2/R3 homologues from the
MLB locus
6
5
1
4
3
0.1 substitution/site
2
GP185 region
GP250 region