Transcript Document

Whole-genome oligonucleotide microarray expression data for all predicted gene models in P. trichocarpa. Values represent the
proportion of genes expressed above negative controls at a 5% false discovery rate. The x axis represents the subsets of predicted
genes that were analyzed for the annotated and promoted P. trichocarpa gene set (42,373 genes), chloroplast gene set (49 genes),
mitochondria gene set (49 genes), annotated, nonpromoted gene set (10,875 genes), and microRNAs (48 miRNAs).
Chromosome-level reorganization of the most recent genome-wide duplication event in
Populus. Common colors refer to homologous genome blocks, presumed to have arisen
from the salicoid-specific genome duplication 65 Ma, shared by two chromosomes.
Chromosomes are indicated by their linkage group number (I to XIX). The diagram to the
left uses the same color coding and further illustrates the chimeric nature of most linkage
groups.
The 4DTV metrics for paralogous gene pairs in Populus-Populus
and Populus-Arabidopsis. Three separate genome-wide
duplications events are detectable, with the most recent event
contained within the Salicaceae and the middle event apparently
shared among the Eurosids. (B) Percent identity distributions for
mutual best EST hit to Populus trichocarpa CDS.
Fig. 4. Kolmogorov-Smirnov (K-S) test for differential expression for 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase genes [for descriptions of the EST data set, see Sterky
et al. (79)]. Results suggest that the duplicated genes in Populus are differentially expressed in
alternate tissues. Tissue types include: cambial zone (1), young leaves (2), flower buds (3), tension
wood (4), senescing leaves (5), apical shoot (6), dormant cambium (7), active cambium (8), cold
stressed leaves (9), roots (10), bark (11), shoot meristem (12), male catkins (13), dormant buds (14),
female catkins (15), petioles (16), wood cell death (17), imbibed seeds (18) and infected leaves (19).
Proportion of eurosid and salicoid duplicated gene sets differentially expressed in
stems (nodes and internode), leaves (young and mature), and whole roots.
Samples from four biological replicates collected from the reference genotype
Nisqually 1 were individually hybridized to whole-genome oligonucleotide
microarrays containing three 60-oligomer oligonucleotide probes for each gene.
Differential expression between duplicated genes was evaluated in t tests and
declared significant at a 5% false discovery rate
Phylogenetic analysis of gene families in Populus, Arabidopsis, and Oryza encoding selected lignin
biosynthetic and related enzymes. (A) Cinnamate-4-hydroxylase (C4H) gene family. (B) 4-coumaroylshikimate/quinate-3-hydroxlase (C3H) gene family. (C) Cinnamyl alcohol dehydrogenase (CAD) and
related multifunctional alcohol dehydrogenase gene family. Arabidopsis gene names are the same as
those in Ehlting et al. (80). Populus and Oryza gene names were arbitrarily assigned; corresponding gene
models are listed in table S13. Genes encoding enzymes for which biochemical data are available are
highlighted with a green flash. Yellow circles indicate monospecific clusters of gene family members.