Transcript WWMD
Mendel’s Contribution(s)?
Finish C. elegans experiment,
This Week
Tinker with wasps,
Exam Review (April 20th)
Exam I (April 23rd),
Basic Mendel!
Basic Mendel!
Basic Mendel!
Basic Mendel!
Basic Mendel!
C. elegans!
Bioinfomatics!
Nasonia!
Mendel’s First Postulate
Unit Factors in Pairs
Genetic characteristics are controlled by unit factors
that exist in pairs* in individual organisms,
each individual receives one unit factor from each
parent*,
in a monohybrid cross, three combinations of unit
factors are possible,
* not in Nasonia males!
Mendel’s Second Postulate
Dominant/Recessive
When two unlike unit factors are present in a single
individual, one unit factor is dominant to the other,
which is said to be recessive.
Mendel’s Third Postulate
Segregation
During the processes of heredity, the paired unit
factors separate so that the offspring receives one
unit factor from each parent,
The unit factors segregate to offspring randomly.
Mendel’s Forth Postulate
Independent Assortment
How do two traits segregate in the offspring of an
individual that is heterozygous for both traits?
Independent Assortment
…checking complementation in the F2
P:
AAdd x aaDD
P:
aadd x AADD
F1:
AaDd
F1:
AaDd
F2:
9: A_D_
F2:
9: A_D_
3: aaD_
3: aaD_
3: A_dd
3: A_dd
1: aadd
1: aadd
Both Recessive?
Both Dominant?
9:7 wt : mut
1:15 wt : mut
One of each?
3:13 wt : mut
P to F1
Haplo/Diplo: Thinking about complementation groups…
1. Both recessive:
aaDD x Ad female: AaDd, male aD
2. Both Dominant: aaDD x Ad female: AaDd, male aD
3. One dominant*: aadd x AD female: AaDd, male ad
or…
AADD x ad female: AaDd, male AD
* need to define which is dominant!
P to F1 to “F2”
Haplo/Diplo: Thinking about complementation groups…
1. Both recessive: aaDD x Ad female: AaDd ¼AD + ¼Ad + ¼aD + ¼ad
2. Both Dominant: aaDD x Ad female: AaDd ¼AD + ¼Ad + ¼aD + ¼ad
3. One dominant*: aadd x AD female: AaDd ¼AD + ¼Ad + ¼aD + ¼ad
or…
AADD x ad female: AaDd ¼AD + ¼Ad + ¼aD + ¼ad
* need to define which is dominant!
Forked-Line Method
Haplo/Diplo F2
AaDd x AD* (*male genotype could be ad, aD, Ad)
1/2 A
1/2 a
½D
1/2 x 1/2 = 1/4 AD
1 AD = ¼ AADD
½d
1/2 x 1/2 = 1/4 Ad
1 AD = ¼ AADd
1/2 D
1/2 x 1/2 = 1/4 aD
1 AD = ¼ AaDD
½d
1/2 x 1/2 = 1/4 ad
1 AD = ¼ AaDd
Forked-Line Method
Haplo/Diplo F2
AaDd x AD* (*male genotype could be ad, aD, Ad)
1/4 AD x 1 AD = ¼ AADD
1/4 AD x 1 ad = ¼ AaDd
1/4 Ad x 1 AD = ¼ AADd
1/4 Ad x 1 ad = ¼ Aadd
1/4 aD x 1 AD = ¼ AaDD
1/4 aD x 1 ad = ¼ aaDd
1/4 ad
x 1 AD = ¼ AaDd
1/4 ad
x 1 ad = ¼ aadd
Have Fun!
1/4 AD x 1 aD = ¼ AaDD
1/4 AD x 1 Ad = ¼ AADd
1/4 Ad x 1 aD = ¼ AaDd
1/4 Ad x 1 Ad = ¼ AAdd
1/4 aD x 1 aD = ¼ aaDD
1/4 aD x 1 Ad = ¼ aaDd
1/4 ad
x 1 aD = ¼ aaDd
1/4 ad
x 1 Ad = ¼ Aadd
Lab
Life Cycles
Wasp Development
Timelines
Life Cycles, Mating, etc.
C. elegans
Nomenclature
Tower of Babel
Wednesday,
This Week
score F3s,
discuss Mendel Review
Formal Lab Write-Up,
think about or tinker with
wasps?
Friday,
midterm,
think about or tinker with
wasps?