Transcript Document

Differential Gene Expression in Olympia Oyster (Ostrea lurida) Gonadal Tissue
Hannah Wear, Carolyn Friedman, and Steven Roberts
School of Aquatic and Fishery Sciences, University of Washington
Differential Gene Expression in Male compared to Female
Background
Transcriptomic data provides information which can aid in conservation
of species with high vulnerability, such as the Olympia oyster.
Research Objectives: Expand existing annotated O. lurida
transcriptome resources and determine which genes have the
greatest difference in expression between O. lurida gonad tissue.
The DESeq analysis figure shows the fold change of male
expression levels, where positive fold change indicates a gene
expressed higher in male gonad tissue, and vice versa. DESeq
analysis indicates 1789 genes expressed significantly higher in
female gonad tissue and 495 genes expressed significantly
higher in male gonad tissue. Red dots represent differentially
expressed genes with a significant p-value (<0.05).
Biological Processes
Approach
Enrichment of Genes and Pathways
1. Transcriptome Annotation: BLAST O. lurida contigs to known gene sequences using multiple gene
and protein databases and assess results with gene ontology (GO) terms.
2. Expression Analysis: Use DESeq analysis to determine differentially expressed contigs from O.lurida
male and female gonad tissue.
Cellular Components
Genes with significant differential expression from DESeq
analyses were subjected to enrichment analysis using DAVID
analysis and categorized by GO terms into three categories:
biological processes, cellular components, and molecular
function. Results also showed significant differential
expression in the N-glycan biosynthesis pathway.
O. lurida Transcriptome Annotation
Molecular Function
GO enrichment of significantly expressed genes
Gene Ontology terms associated with UniProt/Swissprot annotations
Cellular Components
Biological Processes
Molecular Function
Conclusions
These findings and tools expand the existing public genomic resources on O. lurida
and provide baseline data for conservation of the Olympia oyster and related species.
Biological Processes
Cellular Components
Molecular Function
Project funded in part by a NOAA Aquaculture Program Award (NA10OAR4170057) and SAFS.